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Korean J Parasitol > Volume 24(1):1986 > Article

Original Article
Korean J Parasitol. 1986 Jun;24(1):42-48. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1986.24.1.42
Copyright © 1986 by The Korean Society for Parasitology
The karyotype of Paramphistomum explanatum (Creplin, 1849) obtained from Korean cattle
Jae Ku Rhee,Chang Won Kang and Ho Il Lee
Department of Veterinary Parasitology, Jeonbug National University, Korea.

As a series of systematic classification of paramphistomes, in the first step, paramphistomes in the rumen and reticulum were collected on 170 Korean cattles (2-3 years age, male) slaughtered at Jeonju abattoir from July 1984 to September 1985 and were classified by means of morphology of the worms. Afterwards, the karyotype of Paramphistomum explanatum (Creplin, 1849) which is the common in Korean cattle was detected by means of modified air-drying method from testis cells of the worm. The following is a brief summary of the leading facts gained through the experiment. Most of the cattle slaughtered at the abattoir were infected with paramphistomes. The 5 species of the worms were detected on 170 Korean cattle and the worm burden per head was from 2 to 784 (on the average 170) worms, 120(70.59 percent) heads out of them involving 2-100 worms. In 28,900 individuals of paramphistomes obtained on 170 Korean cattle, appearance rates of various worms were as follows : 49.74 percent in P. explanatum, 48.08 percent in P. cervi, 0.98 percent in Orthocoelium orthocoelium, 0.89 percent in Fischoederius cobboldi and 0.14 percent in Cotylophoron cotylophorum. The chromosome number of 620 P. explanatum in the haploid and diploid cells was n=9 and 2n=18, and abundant cells in meiotic division were observed; 1,420 haploid and 38 diploid cells were reliable. Nine pairs of mitotic chromosomes were homologous and the chromosomes were composed of five medium-sized metacentrics (m), subtelocentrics (st) or submetacentrics (sm)and four small-sized subtelocentrics (st) or submetacentrics (sm), while meiotic metaphase chromosomes were composed of five medium and four small-sized. The haploid of the testis cells showed C-band in the centromeric region from 8 of them, whereas the remaining chromosome No. 5 included heterochromatin on the tip region, and chromosomes No. 3 and No. 7 showed a remarkable C-band distinguished from other chromosomes.


Fig. 1
The mitotic chromosomes(10) in cultured testis cells of Paramphistomun explanatum prepared by modified air dry method.

Fig. 2
The meiotic chromosomes(9) in cultured testis cells of Paramphistomum explanatum prepared by modified air dry method.

Fig. 3
The karyotype plate made from the mitotic metaphase of Paramphistomum explanatum.

Fig. 4
The meiotic chromosomes (haploid) of Paramphistomum explanatum.

A: Conventional karyotype of testis cell B: C-banded karyotype of testis cell.


Table 1
Measurements of various organ of paramphistomes obtained from Korean cattle

Table 2
Appearance rates of various paramphistomes obtained from 170 Korean cattle

Table 3
Worm burden of paramphistomes in 170 Korean cattle

Table 4
Chromosome measurements and their classification of Paramphistomum explanatum

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