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Korean J Parasitol > Volume 31(1):1993 > Article

Original Article
Korean J Parasitol. 1993 Mar;31(1):49-56. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.1.49
Copyright © 1993 by The Korean Society for Parasitology
Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis
Jong Hyun Lee,Yoon Kong,Jae-Young Ryu and Seung-Yull Cho*
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Received December 19, 1992; Accepted January 26, 1993.


Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has widely been applied. This study was undertaken to observe whether diagnostic capability can be improved by applying more sensitive techniques such as Protein A-ELISA and avidin biotin complex ELISA (ABC-ELISA). When evaluated using 115 sera of human cysticercosis, the antibody positive rates were not significantly improved in Protein A-ELISA (82.6%) and in ABC-ELISA (86.1%) than in micro-ELISA (81.7%). The specificities, evaluated in 165 sera from other diseases and normal controls, were significantly improved (88.5% by micro-ELISA, 93.3% by Protein A-ELISA and 93.8% by ABC-ELISA). Antibody levels (absorbance, abs.) in individual serum were correlated well (r = 0.83-0.86) each other. An actual benefit of Protein A-ELISA and ABC-ELISA was that they needed smaller amount of test sample.


Fig. 1
Relations of the individual antibody levels (abs.) as measured by micro-ELISA and Protein A-ELISA in sera of 115 cysticercosis patients. Horizontal and vertical dotted lines of abs. 0.18 or 0.10 are the positive criteria.

Fig. 2
Relations of the individual antibody levels (abs.) as measured by micro-ELISA and ABC-ELISA in sera of 115 cysticercosis patients. Dotted lines at abs. 0.18 and 0.10 are the same as described in Fig. 1.

Fig. 3
Distribution and relation of the specific antibody levels (abs.) between micro-ELISA and Protein A-ELISA to T. solium metacestode antigen in 165 sera of other diseases and normal controls. Horizontal and vertical lines of abs. 0.18 and 0.10 are the positive criteria.

Fig. 4
Distribution and relation of the specific antibody levels (abs.) between micro-ELISA and ABC-ELISA to T. solium metacestode antigen in 165 sera of other diseases and normal controls. Lines and symbols are the same as described in Fig. 3.


Table 1
Procedures and conditions of 3 different ELISA techniques employed in this study

Table 2
Results of specific-antibody tests by 3 different techniques of ELISA in each category of patients

Table 3
Sensitivity and specificity of three techqniques of ELISA as calculated in 115 cysticercosis and 165 other diseases and normal control

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