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Korean J Parasitol > Volume 33(4):1995 > Article

Original Article
Korean J Parasitol. 1995 Dec;33(4):377-382. English.
Published online Dec 20, 1995.  http://dx.doi.org/10.3347/kjp.1995.33.4.377
Copyright © 1995 by The Korean Society for Parasitology
Oocyst production and immunogenicity of Cryptosporidium muris (strain MCR) in mice
Jae Ku Rhee,*1Sim Young Yook,1 and Bae Keun Park2
1Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju 561-756, Korea.
2Department of Parasitology, School of Veterinary Medicine, Chungnam National University, Taejon 305-764, Korea.
Received September 30, 1995; Accepted November 07, 1995.


Three-week-old ICR SPF mice were orally inoculated with one of 5 doses ranging from 2 × 102 to 2 × 106 oocysts of Cryptosporidium muris (strain MCR) per mouse. Oocyst inoculation was directly proportional to the amount of oocysts shed and was inversely proportional to the period required for peak oocyst production and to the prepatent period. Peak oocyst production occurred between fifteen and thirty-one days with a patent period from 61 to 64 days. Three days after all mice stopped shedding oocysts, they were orally challenged with a single dose of 2 × 106 oocysts of the same species. Marked seroconversion for IgG antibody accompanied recovery from mice inoculated with 5 × 105 oocysts. Mice administered with carrageenan excreted a small number of oocysts for 49.0 days on the average after challenge inoculation (ACI) and control mice for 14.2 days in a dose-independent fashion. Just before challenge infection, phagocytic activity of peritoneal macrophages (Mø) and the number of peripheral Mø were dramatically decreased. Mild challenge infection implies that the immunogenicity of C. muris (strain MCR) is very strong, despite Mø blocker carrageenan administration.


Table 1
Mean daily oocyst productiona) in mice inoculated with Cryptosporidium muris (strain MCR)

Table 2
Mean daily oocyst production in mice after primary and challenge inoculations with Cryptosporidium muris

Table 3
Effect of carrageenan on the number of Mø in peripheral blood and T-cells in splenocytes and on the phagocytic activity of peritoneal Mø in mice

1. Campbell PN, Current WL. Demonstration of serum antibodies to Cryptosporidium sp. in normal and immunodeficient humans with confirmed infections. J Clin Microbiol 1983;18(1):165–169.
2. Hsu SM, Raine L, Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 1981;75(5):734–738.
3. Iseki M, Maekawa T, Moriya K, Uni S, Takada S. Infectivity of Cryptosporidium muris (strain RN 66) in various laboratory animals. Parasitol Res 1989;75(3):218–222.
4. Maekawa T. J Osaka City Med Cen 1988;37(3):679–699.
5. Matsui T, et al. Jpn J Parasitol 1994;43(3):199–204.
8. Rhee JK, Seu YS, Park BK. [Isolation and identification of Cryptosporidium from various animals in Korea. I. Prevalence of Cryptosporidium in various animals]. Korean J Parasitol 1991;29(2):139–148.
9. Rhee JK, Seu YS, Park BK. [Isolation and identification of Cryptosporidium from various animals in Korea. II. Identification of Cryptosporidium muris from mice]. Korean J Parasitol 1991;29(2):149–159.
10. Rhee JK, Jang BG, Park BK. Oocyst production and immunogenicity of Cryptosporidium baileyi in chickens and mallards. Korean J Parasitol 1995;33(1):45–54.
11. Tyzzer EE. Proc Soc Exp Biol Med 1907;5:12–13.
12. Tyzzer EE. J Med Res 1910;23:487–509.
13. Tyzzer EE. Arch Proristenkd 1912;26:394–412.
14. Ungar BL, Mulligan M, Nutman TB. Serologic evidence of Cryptosporidium infection in US volunteers before and during Peace Corps service in Africa. Arch Intern Med 1989;149(4):894–897.
15. Upton SJ, Current WL. The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals. J Parasitol 1985;71(5):625–629.
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