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Korean J Parasitol > Volume 21(1):1983 > Article

Original Article
Korean J Parasitol. 1983 Jun;21(1):11-20. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1983.21.1.11
Copyright © 1983 by The Korean Society for Parasitology
Experimental Infection of Clonorchis sinensis to Cyprinus carpio nudus
Jae Ku Rhee,Pyung Gil Kim,Byeong Kirl Baek,Sang Bork Lee and Byung Zun Ahn
Department of Veterinary Medicine, Jeonbug National University, Korea.
Department of Pharmacy, Chungnam National University, Korea.
Abstract

As a part of observation on the suitability as a second intermediate host of Clonorchis sinensis, various infection experiments were tried to Cyprinus carpio nudus. The results obtained were summarized as follows:

1. The cercariae which attempted to contact with Cyprinus carpio nudus in the water were observed under the stereomicroscope. After contact, cercariae began to separate its tails from the bodies at 4 minutes increasingly, and then the number ranged to 80 percent at 13 minutes after the encounter. But very few cercariae could actually invade into the epidermis of the fish.

2. When Cyprinus carpio nudus were exposed to a number of cercariae in the beaker, only a few cercariae could invade through the epidermis. Most of the invaded cercariae were killed before forming the cyst. Also rare encysted cercariae were found to be dead within 48 hours.

3. Cyprinus carpio nudus were reared with Parafossarulus manchouricus shedding numerous cercariae in the indoor aquarium. When observed after 12 days, to determine the susceptibility, no metacercaria was found infected.

4. Cyprinus carpio nudus were reared with Parafossarulus manchouricus shedding numerous cercariae in Kimhae lake for 2 months in summer of 1982, in order to check the natural infection. No metacercaria of Clonorchis sinensis was found from the fish.

5. In the cases of control fish, Pseudorasbora parva, numerous cercariae were inspected on the course of invasion through epidermis of the fish under the stereomicroscope. And many metacercariae were also found from the fish whenever they were kept in beaker, indoor aquarium, or the lake for natural infection.

The results suggested that Cyprinus carpio nudus might not be proper intermediate host of Clonorchis sinensis.

Figures


LEGEND FOR FIGURES
Figures given are of the cercaria or the metacercaria of Clonorchis sinensis which is detected from Cyprinus carpio nudus, and the magnification is ×400, from figure 3 to 8.

Fig. 1. Cylindrical raising net is attached to empty plastic pail in order to float the net.

Fig. 2. Cylindrical raising net is established in Kimhae lake.

Fig. 3. The live metacercaria which is detected in the caudal muscle is 120×50µm in size, and its eye spots and oral sucker are visible although other internal structures are obscure. 6hours after infection.

Fig. 4. Degenerated dead cercaria is found in the dorsal muscle, and its eye spots and large yellowish brown pigment masses are distinct, though its body is depressed on the middle part. 6 hours after infection.

Fig. 5. Degenerated dead metacercaria which is found in the dorsal muscle has partially formed cyst wall and its eye spots with yellowish brown pigment masses scattered over its wall boundary. 18 hours after infection.

Fig. 6. Two dead precystic larvae which are observed in the epidermis have the size of around 120×50µm and wrinkled body wall with their eye spots and yellowish brown pigment masses, whereas their other internal organs are obscure. 18 hours after infection.

Fig. 7. The live metacercaria which is found in the caudal fin is 130×30µm in size and shows slight bend in its meddle part with its eye spots and oral sucker. 24 hours after infection.

Fig. 8. The metacercaria which has less vigorous motility is observed in the caudal fin with its eye spots and oral sucker. 24 hours after infection.


Tables


Table 1
Result of experimental infection with Clonorchis sinensis cercariae to Cyprinus carpio nudus and Pseudorasbora parva in 1 L beaker


Table 2
Result of experimental infection with Clonorchis sinensis cercariae to Cyprinus carpio nudus and Pseudorasbora parva in indoor aquarium


Table 3
Result of natural infection experiment with various cercariae to Pseudorasbora parva in Kimhae lake*


Table 4
Result of natural infection experiment with various cercariae to Cyprinus carpio nudus in Kimhae lake

References
1. Shin DS. [Epidemiological studies of Clonorchis sinensis prevailed in the peoples of Kyungpook Province]. Korean J Parasitol 1964;2(1):1–13.
 
2. Rhee JK. [Experimental studies on the second intermediate hosts of Clonorchis sinensis: III. Observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of Clonorchis sinensis in fresh-water fish]. Korean J Parasitol 1974;12(2):101–110.
 
3. Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: I Preliminary Research On The Wormicidal Substance From Mucous Substance Of Carassius Carassius]. Korean J Parasitol 1979;17(2):121–126.
 
4. Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: II. Preliminary Research On The Wormicidal Substances From Mucous Substances Of Various Fresh Water Fishes]. Korean J Parasitol 1980;18(1):98–104.
 
5. Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: III. Seasonal variation on the wormicidal substances of mucous substances of Carassius carassius]. Korean J Parasitol 1980;18(2):179–184.
 
6. Rhee JK, Kim PG, Baek BK, Lee SB, Ahn BZ. Clavate Cells Of Epidermis In Cyprinus Carpio Nudus With Reference To Its Defence Activity To Clonorchis Sinensis. Korean J Parasitol 1982;20(2):201–203.
 
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