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Korean J Parasitol > Volume 20(1):1982 > Article

Original Article
Korean J Parasitol. 1982 Jun;20(1):14-20. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1982.20.1.14
Copyright © 1982 by The Korean Society for Parasitology
The detectability of helminth eggs from feces by cellophane thick smear technique
Jong-Yil Chai,Young-Tae Yang,Soon-Hyung Lee and Byong-Seol Seo
Basic Medical Research Section, Seoul District Armed Forces General Hospital, Korea.
Department of Parasitology, College of Medicine,Seoul National University, Korea.

The efficacy of the cellophane thick smear(CTS) technique was reassessed for detecting helminths eggs such as Ascaris lumbricoides, Trichuris trichiura and Clonorchis sinensis, in comparsion with the formalin-ether(F-E) one, with repeated smears per case. From the fecal samples of 411 ROK Army soldiers, a total of 3,288 smears were made and examined; 4 by CTS and 4 by F-E techniques. The expected egg detectability with one, two, three or four smears by each technique was mathemativally calculated.

The results were as follows:

1. When the results of all 8 smears per case were integrated, the number of cases eith Ascaris egg were 54(13.1%), Trichuris 125(30.4%) and Clonorchis 33(8.0%). Some other kinds of helminths eggs and protozoan cysts were found from a few cases each.

2. The calculated detectability of Ascaris eggs by CTS technique was fairly high(95.8~96.3%) with 1-4 smears, while that by F-E technique was not so high(73.1~88.9%).

3. In case of Trchuris eggs, the egg detectability by CTS technique with 1-4 smears was a little lower(62.2-86.4%) than that by F-E technique(73.4 - 91.2%).

4. The detectability of Clonorchis eggs with 1-4 smears by CTS technique was the lowest(44.7~60.6%) among the three helminths as well as much lower than that by F-E technique(67.4~87.9%).


Fig. 1
The graphic view of the calculated egg detectability according to examination techniques and number of smears. ○-○: cellophane thick smear technique, ●-●: formalin-ether technique.


Table 1
The integrated results of stool examination both by CTS and F-E techniques with 8 smears per case

Table 2
The detailed results of examinations on the egg-producers by CTS and F-E techniques with a total of 8 smears per case

Table 3
The calculated egg detectability according to methods and number of smears to be examined

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