| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasitol > Volume 19(1):1981 > Article

Original Article
Korean J Parasitol. 1981 Aug;19(1):1-7. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1981.19.1.1
Copyright © 1981 by The Korean Society for Parasitology
A comparative analysis of various parts of Ascaris suum with respect to their protein composition
Won-Young Choi,Young-Kwan Jin,Ok-Ran Lee and Woon-Gyu Kim
Department of Parasitology, Catholic Medical College, Korea.
Abstract

For the purpose of making a comparative study of protein compositions in Ascaris suum by sexes and body parts, extracts were prepared from whole bodies, body walls, genital organs, digestive organs and body fluid, of both sexes. And electrophoretic analysis was conducted using polyacrylamide slab gel in the presence of sodium dodecyl sulfate.

The results were as follows:

1. In this study, protein bands of each part were separated in the largest number and most clearly under 8%~12% (10%) gradient gel condition. The number of bands by body parts was 43 in body walls, 51 in genital organs, 47 in digestive organs, and 34 in body fluid.

2. When examined in terms of sex, the number of bands of whole body was 38 in females and 35 in males.

3. The electrophoretic patterns of body wall protein were in most cases similar with those seen in digestive organs. And the band with a molecular weight of 72,900 was unique to the body wall, and the 122,000 MW band was unique to the female body wall.

4. In genital organ extracts, large molecular weight proteins (more than 80,000) were more frequently met. The molecular weights showed some differences between the two sexes. Of the bands, those having molecular weights of 119,700, 100,500, 88,500 and 86,100 were unique to the female genital organs. On the other hand, the male genital organs showed unique bands having molecular weights of 87,100, 82,800, and 15,500. An unique band common to the genital organs of both sexes was one having 49,300 MW.

5. In the digestive organs evenly distributed protein bands of molecular weights of l0,000~120,000 were observed. The band having 59,800 MW was unique to the digestive organs.

6. The number of bands obtained from body fluid was comparatively small, and the number of bands having less than 30,000 MW was 7, accounting for 55% of the total protein amounts. The bands having 47,600 MW and 31,400 MW were unique to body fluid.

Figures


Fig. 1
Electrophoretic mobility of standard proteins as a function of molecular weight in 10% acrylamide slab gels.


Fig. 2
10% acrylamede slab gels of Ascaris suum of different part of body and sex.

M: male, F: female 1~4: standard protein

1: LDH (35,000MW), 2: G-3-PD(36,000MW), 3: aldolase(40,000MW), 4: B.S.A.(67,000MW), 5: whole worm (M), 6: whole worm (F), 7: cuticule mescle (M), 8: cuticule muscle (F), 9: genital organ (M), 10: genital organ (F), 11: intestine (M), 12: intestine (F), 13: coelomic fluid (M), 14: coelomic fluid (F)

A,B,C,D & E means the bands ubiquitously found in all samples.



Figs. 3-12
Densitometric analysis of electrophoretic patterns of Ascaris suum.

Fig. 3. Whole female extracts.

Fig. 4. Whole male extracts.

Fig. 5. Female cuticle and muscle extract.

Fig. 6. Male cuticle and muscle extracts.

Fig. 7. Female genital organ extracts.

Fig. 8. Male genital organ extracts.

Fig. 9. Female intestine extracts.

Fig. 10. Male intestine extracts.

Fig. 11. Female coelomic fluid.

Fig. 12. Male coelomic fluid.


References
1. Abbas M, Foor WE. Ascaris suum: free amino acids and proteins in the pseudocoelom, seminal vesicle, and glandular vas deferens. Exp Parasitol 1978;45(2):263–273.
  
2. Ambler J, et al. Immunology 1973;10:815–820.
3. Choi WY, Lee OR. [Immunoelectrophoretic Studies Of Several Helminths]. Korean J Parasitol 1979;17(2):147–153.
 
4. Jeska EL. Antigenic analysis of a metazoan parasite, Toxocara canis. II. Purification and analysis of two antigenic components. J Immunol 1967;98(6):1290–1300.
 
5. Jeska EL. Purification and immunochemical analysis of a genus-specific cuticular antigen of Toxocara canis. J Parasitol 1969;55(3):465–471.
  
6. Huntley CC, Moreland A. Gel diffusion studies with Toxocara and Ascaris extracts. Am J Trop Med Hyg 1963;12:204–208.
 
7. Hussain R, et al. J Immun 1973;111:1.
8. Kagan IG. Serum-agar double diffusion studies with ascaris antigens. J Infect Dis 1957;101(1):11–19.
  
9. Kagan IG, Jeska EL, Gentzkow CJ. Serum-agar double diffusion studies with Ascaris antigens. II. Assay of whole worm and tissue antigen complexes. J Immunol 1958;80(5):400–406.
 
10. Kent HN. Exp Parasit 1960;10:313–323.
 
11. Kuo CY, et al. Int Archs Allergy appl Immun 1977;54:308–314.
 
12. Kurimoto H. Jpn J Parasit 1974;23(5):255–258.
13. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
 
14. O'Donnel IJ, et al. Aust J Biol Sci 1978;1:459–487.
15. Sodeman WA Jr. Studies on the protein composition of extracts of Schistosoma mansoni cercariae. J Parasitol 1968;54(4):775–779.
  
16. Soulsby EJL. Trans Roy Soc Trop Med Hyg 1957;51:9–10.
17. Studier FW. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol 1973;79(2):237–248.
  
18. Tormo BJ, et al. Nature 1965;25:983–985.
 
19. Williams JF, Soulsby EJ. Antigenic analysis of developmental stages of Ascaris suum. I. Comparison of eggs, larvae and adults. Exp Parasitol 1970;27(1):150–162.
  
20. Yoshida Y, et al. Jpn J Parasit 1977;26(3):168–174.
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
  E-Mail
Share:      
METRICS
0
Crossref
0
Scopus
2,644
View
26
Download
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2022 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI