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Korean J Parasitol > Volume 18(2):1980 > Article

Original Article
Korean J Parasitol. 1980 Dec;18(2):185-191. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1980.18.2.185
Copyright © 1980 by The Korean Society for Parasitology
In vitro activities of various drugs against adult worms of Clonorchis sinensis
Han-Jong Rim,Sung-Whan Jo,Kyoung-Hwan Joo and Sang-Soon Kim
Department of Parasitology and Institute for Tropical Endemic Diseases, College of Medicine, Korea University, Korea.
Abstract

The present study was undertaken to evaluate in vitro activities of following drugs against adult worms of Clororchis sinensis: i.e., stibnal, chloroquine diphosphate, Hetol (1,4-bis-trichloromethylbenzol), dehydroemetine 'Roche' (Ro 1-9334), niridazole (Ambilhar), bisbendazole, gentian violet, dithiazanine iodide, hexachlorophene, bithionol, niclofolan (Bilevon, Bayer 9015) and praziquantel (Embay 8440, Biltricide).

After isolation from bile ducts of experimentally infected rabbits (3 months infection), the parasites were rinsed in sterilized Tyrode's solution and incubated for 24 hours at 37℃ in TC 199 medium with Earle's balanced salt solution containing 0, 0.01, 0.1, 1.0, 10 and 100 µg/ml concentrations of each above drugs. The activities of each concentrations with each drugs were evaluated by the motility of the worms in culture tubes observing at 15, 30, 60 minutes and 2, 4, 6, 12, 18 and 24 hours by stereomicroscope.

In the results, no effect was found at the adult worms of C. sinensis in all concentrations with stibnal, chloroquine diphosphate, Hetol, dehydroemetine, niridazole and bisbendazole. However, moderate activities were observed in the concentrations of 1.0 and 0.1 µg/ml of gentian violet, dithiazanine iodide, hexachlorophene and bithionol. The highest activities were observed in all concentrations of niclofolan and praziquantel.

Tables


Table 1
Effects of various drugs against the adult worms of Clonorchis sinensisin vitro


Table 2
In vitro activities of gentian violet and dithiazanine iodide against adult worms of Clonorchis sinensis


Table 3
In vitro activities of hexachlorophene and bithionol against adult worms of Clonorchis sinensis


Table 4
In vitro activities of niclofolan and praziquantel against adult worms of Clonorchis sinensis

References
1. Chu HJ. Chinese Med J 1938;54:409–415.
2. Koyama H. Jpn J Parasit 1967;16:344–368.
3. Rim HJ, Chang DS, Hyun I, Song SD. Effectiveness of anthelmintic drugs against Clonorchis sinensis infection of rabbits. Korean J Parasitol 1975;13(2):123–132.
 
4. Rim HJ, Ha JH, Kim SJ. [Experimental study on the therapeutic effect of praziquente1(Embay 8440) in rats experimentally infected with Clonorchis sinensis]. Korean J Parasitol 1980;18(1):65–80.
 
5. Rim HJ, et al. Korea Univ Med J 1976;13:197–204.
6. Rim HJ, et al. Korea Univ Med J 1979;16:167–182.
7. Yamaguchi T, et al. Naika No Ryoiki 1961;9:258–263.
8. Yokogawa M, et al. Jpn J Parasit 1965;14:233–242.
9. Yokogawa M, et al. Jpn J Parasit 1965;14:526–533.
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