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Korean J Parasitol > Volume 18(2):1980 > Article

Original Article
Korean J Parasitol. 1980 Dec;18(2):179-184. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1980.18.2.179
Copyright © 1980 by The Korean Society for Parasitology
The wormicidal substances of fresh water fishes on Clonorchis sinensis III. Seasonal variation on the wormicidal substances of mucous substances of Carassius carassius
Jae Ku Rhee,Byeong Kirl Baek,Byung Zun Ahn and Young Jun Park
Department of Veterinary Medicine, College of Agriculture, Jeonbuk National University, Korea.
Korea Research Institute of Chemical Technology, Korea.
Department of Veterinary Medicine, College of Agriculture, Jeonnam National University, Korea.
Abstract

The epidermal mucous substances of C. carassius which were extracted by ether were separated into various spots on the silica gel thin layer chromatography and separated into various fractions in the silica gel column chromatography, using petroleum ether (30%) and chloroform(70%) as a solvent.

The wormicidal effects of the spots and the fractions on the cercaria, the liberating metacercaria and the adult of C. sinensis, were observed by month giving attention to ambient water temperature.

1. Among the four spots of the thin 1ayer chromatography of the ether extracts from C. carassius, the uppermost first spots (Rf. 0.924~0965) had a strong wormicidal effect on the cercaria in May, June, July, August and September, and the cercaria which came in contact with the materials was killed in 29~49 min., but in other months (Rf. 0.612~0.752) it was killed only after 50-115 min.

2. In column chromatography of ether extracts from C. carassius, the first fractions which had a wormicidal effect killed the metacercariae within 38~163 min. in May, June, July, August, and September, but it took about 120~458 min. to kill them in other months, meanwhile the wormicidal time of the fractions on the adults was 445~835 min. in May, June, July, August and September, but was 878-2,130 min. in other months.

3. The Rf. value of the uppermost first spot of the thin layer chromatography of ether extrart from the mucous substance of C. carassius which was reared in the aquarium controlled at 23℃ was 0.79, and its first fraction of column chromatography killed the metacercaria and the adult of C. sinensis within 315, and 1,850 min. respectively.

As summarized above, the wormicidal materials of the epidermal mucous substances of C. carassius had no relation to ambient water temperature, but the secretion period of this wormicidal materials was the same as the liberating period of the cercariae from Parafossarulus manchouricus.

Figures


Fig. 1
The time for wormicide of the first spots on the thin layer chromatography of the ether extracted mucous substances from C. carassius on the cercariae of C. sinensis.

Ŷ=108.168-3.3288χ-3.089χ2+0.2848χ3

R=0.868 dŷ/dχ ; χ=7.7346 Ŷ=29.406



Fig. 2
The time for wormicide of the first fractions in column chromatography of the ether extracted mucous substances from C. carassius on the metacercariae of C. sinensis.

Ŷ=494.192-120.338χ+5.241χ2+0.378χ3

R=0.868 dŷ/dχ=0 ; χ=6.6689 Ŷ=36.8718



Fig. 3
The time for wormicide of the first fractions in column chromatography of the ether extracted mucous substances from C. carassius on the adults of C. sinensis.

Ŷ=2349.92-220.49χ-40.27χ2+4.76χ3

R=0.751 dŷ/dχ=0 ; χ=7.76567 Ŷ=424.04


Tables


Table 1
Seasonal changes of the Rf. values of spots on the TLC of the ether extracts from mucous substances of C. carassius


Table 2
Seasonal changes of wormicidal effects of the first spots on the TLC of the ether extracts from mucous substances of C. carassius on the cercariae of C. sinensis

References
1. Chung BJ, Joo CY, Choi DW. Seasonal variation of snail population of Parafossarulus manchouricus and larval trematode infection in river Kumho, Kyungpook province, Korea. Korean J Parasitol 1980;18(1):54–64.
 
2. Pfeiffer W. Experimentia 1963;19:113–123.
 
3. Rhee JK. [Experimental studies on the second intermediate hosts of Clonorchis sinensis: III. Observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of Clonorchis sinensis in fresh-water fish]. Korean J Parasitol 1974;12(2):101–110.
 
4. Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: I Preliminary Research On The Wormicidal Substance From Mucous Substance Of Carassius Carassius]. Korean J Parasitol 1979;17(2):121–126.
 
5. Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: II. Preliminary Research On The Wormicidal Substances From Mucous Substances Of Various Fresh Water Fishes]. Korean J Parasitol 1980;18(1):98–104.
 
6. Von Frisch K. Die Naturwissenschaften 1941;29:321–334.
 
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