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Korean J Parasitol > Volume 16(2):1978 > Article

Original Article
Korean J Parasitol. 1978 Dec;16(2):88-102. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1978.16.2.88
Copyright © 1978 by The Korean Society for Parasitology
Ultrastructural changes of the hepatocytes and biliary epithelia due to Clonorchis sinensis in guinea pigs
Sun-Yong Lee,Soon-Hyung Lee and Je G. Chi
Department of Parasitology, College of Medicine, Chung-Ang University, Korea.
Department of Pathology, College of Medicine, Seoul National University, Korea.

The present study was undertaken to observe the changes of hepatocyte and biliary epithelium in the liver of guinea pigs infected with C. sinensis.

Ten guinea pigs infected with 500 metacercariae of C. sinensis each were reared for 11 weeks. After sacrifice, the liver tissues were processed both for conventional light microscopy and for electronmicroscopy.

The following results were obtained by light and electronmicroscopic observation of these tissue preparations.

1. The dilatation of bile ducts with chronic inflammatory cell infiltration and fibrosis, and flattening of lining epithelium were observed by light microscopy. Bile duct showed a single low columnar or cuboidal epithelium and subepithelial small round cell infiltration. Papillary proliferation, adenomatous hyperplasia and increase of goblet cells in the subepithelial glands were also noted in some areas of bile ducts.

Occasionally, small fibrous nodules in the portal tract replacing bile ducts, surrounded by a heavy infiltrate of small round cells and neutrophils were observed.

2. On light microscopic examination, no cellular damages were seen in the liver cells except mild fatty degeneration.

3. Electronmicroscopic examination of the biliary epithelium revealed increased mucin granules, cytoplasmic projection into lumen, decreased microvilli and obstruction of bile canaliculi. Indistinct and/or irregular appearances of intercellular lateral interdigitation were observed in most of biliary epithelium.

4. On the electronmicrographs of hepatocytes, dilation of endoplasmic reticulum(ER) and destruction of cristae in some mitochondriae were prominent features. Disappearance of cytoplasmic organells and dilatation of bile canaliculi were also monitored.

5. Endothelial cells in the sinusoids were remained intact.

Keywords: animal, guinea pig.


Fig. 1
Dilated bile duct harboring a C. sinensis worm in the lumen. The lining epithelium is flattened and the wall of bile duct shows fibrosis and chronic inflammatory infiltrates. (H-E stain, ×40)

Fig. 2
Higher magnification of Fig. 1. It shows a single layer of low columnar or cuboidal epithelia and subepithelial small round cell infiltration. Subepithelial glands show evident mucous metaplasia. (H-E stain, ×100)

Fig. 3
Bile duct epithelium showing papillary proliferation A portion of worm and free floating eggs are seen in the lumen of bile duct. (H-E stain, ×100)

Fig. 4
4Another area of bile duct epithelium showing adenomatous hyperplasia with chronic inflammatory cells and fibrosis in the stroma. (H-E stain, ×100)

Fig. 5
A small fibrous nodule in the portal tract, replacing bile duct, is surrounded by a heavy infiltrate of small round cells and some neutrophil leucocytes. Fatty degeneration of hepatocyte can be seen in remaining parenchyma. (H-E stain, ×40)

Fig. 6
Higher magnification of Fig. 5. Glands show mucous metaplasia and fibrous nodule consists of fibroblasts, capillaries and some small round cells. (H-E stain, ×100)

Fig. 7
Electronmicrograph of normal bile epithelium of control guinea pig. It shows normal architecture of microvilli and much granules. (×5000)

Fig. 8
Normal bile epithelium. Intercellular lateral interdigitation is clearly visible between the adjacent cells.

Fig. 9
Bile epithelium of C. sinensis infected group. Increase of mucin granules in the cytoplasm is prominent.

Fig. 10
Higher magnification of Fig. 9. Increased mucin granules and decrease of microvilli in number are notable.

Fig. 11
Cytoplasmic profection into lumen as well as the decrease of lining microvilli can be seen in bile epithelium of infected group.

Fig. 12
Occulusion of the bile duct.

Fig. 13
Irregular and/or indistinct lateral interdigitation is observed between the adjacent bile epithelial cells of infected group.

Fig. 14
Electronmicrograph of normal hepatocyte. (×5000)

Fig. 15
Hepatocyte of Clonorchis infected group. Extensive dilatation of endoplasmic reticulum is evident.

Fig. 16
Higher magnification of Fig. 15.

Fig. 17
Partial destruction of cristae in the mitochondria is shown in the hepatocyte of Clonorchis infected group.

Fig. 18
The hepatocyte of infected group shows scantiness of cytoplasmic organells.

Fig. 19
Dilation of bile canaliculi between the hepatocytes is seen in Clonorchis infected group.

Fig. 20
In Clonorchis infected group, endothelial cell in the sinusoid remain unaffected.

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