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Korean J Parasitol > Volume 15(2):1977 > Article

Original Article
Korean J Parasitol. 1977 Dec;15(2):75-92. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1977.15.2.75
Copyright © 1977 by The Korean Society for Parasitology
An experimental study on the mouse intraperitoneal inoculation of the adult worm of Clonorchis sinensis
Chul Yong Song,Soon Hyung Lee and Je Geun Chi
Department of Parasitology, College of Medicine, Chung-Ang University, Korea.
Department of Pathology, College of Medicine, Seoul National University, Korea.

The present experimental study was undertaken to observe the chronological change of the worm structure of Clonorchis sinensis and the pathological findings of the liver when this fluke was inoculated to the mouse intraperitoneally.

The recovery rate, survival rate, location and size of the inoculated worms as well as the pathological changes of the liver were investigated for the comparison among the groups of mice, classified by number of worms and the duration of experiment.

The results obtained were summarized as follows.

1. The recovery and survival rates of the worms decreased especially 28 days after the inoculation.

2. Most of worms (90.l%) were collected from the peritoneal cavity and some of worms were found tightly adherent to the capsules of the liver, spleen, stomach, intestine and diaphragm. There were no worms recovered penetrated in the parenchymes of these organs.

3. The mean worm size after inoculation was smaller than that before inoculation. At the 10th day after the inoculation, the shrinkage of posterior portion of the worm body was observed.

4. Remarkable atrophy in the reproductive organs of the worm, such as spermatheca, testes, vitelline glands and ovary was frequently observed at the 10th day of inoculation.

5. Histopathologically the liver failed to show any parasitic worm inside the intrahepatic biliary system. However, multiple well formed egg-containing granulomas were present along the liver capsule. These necrotic granulomas were occasionally found under the fibrotic liver capsule. Focal necrosis and focal phlebitis together with vascular dilatation were prominent features seen in the liver. The bile duct in the liver showed mild dilation of the lumen, flattening of epithelial cells and periductal small round cell infiltration. Neither adenomatous hyperplasia nor portal fibrosis was seen in the whole experimental groups. Foci of intralobular micro-granulomas were found in some experimental animals.

6. The worms recovered in the capsule of the liver were degenerated and necrotized. Usually, there were remarkable capsulitis and granuloma formation around the eggs.


Figs. 1-6
Fig. 1. A normal worm (Clonorchis sinensis) of control group.

Fig. 2. A recovered worm from the abdominal cavity of the mouse, 4 days after the intraperitoneal inoculation. The spermatheca began to atrophy.

Fig. 3. This worm was obtained from the abdominal cavity 10 days after the inoculation. Note the shortened posterior portion of the worm.

Fig. 4. Atrophy of the spermatheca, tests, ovary, vitellaria and scantiness of eggs in the uterus were observed in the worm, 28 days after the inoculation.

Fig. 5. Remarkable atrophy of reproductive organs was visible in this worm, 28 days after the inoculation.

Fig. 6. Elongated or shortened worm bodies were obtained 56days after the inoculation. Note the atrophied reproductive organs.

Figs. 7-10
Fig. 7. Photomicrograph of a necrotic granuloma (presumed to me a dead worm) excavating the liver capsule. H&E ×40.

Fig. 8. Numerous Clonorchis eggs are surrounded by epitheloid granulomas. Langhans giant cell is seen. These granulomas are located just beneath the liver capsule. H&E ×40.

Fig. 9. The liver capsule showing a mild small round cell infiltration (capsulitis) along the surface. H&E ×100.

Fig. 10. Many dilated venous channels are seen. H&E ×40.

Figs. 11-14
Fig. 11. Hepatic lobule showing irregularity of nuclear size. H&E ×400.

Fig. 12. Dilated venous channel is seen in the center, the well of which is infiltrated with various inflammatory cells. The endothelial cells are also involved in the process. H&E ×100.

Fig. 13. Focal necrosis and cell collections including histiocytes are seen around a vein (A). Sometimes these cell collections protrude into the vascular lumen(B). H&E ×100(A) ×400(B).

Fig. 14. Perivascular cell infiltration consists mainly of lymphocytes and histiocytes. H&E ×100(A) ×400(B).

Figs. 15-18
Fig. 15. An area of focal necrosis in a lobule, showing small round cell infiltration alonf with liver cell degeneration. Histiocytes are lacking in this area. H&E ×100.

Fig. 16. Infarction of the liver is seen in the right lower portion.

Fig. 17. An intralobular granuloma with definite epitheloid cell component(A) and its magnification(B). H&E ×100(A) ×400(B).

Fig. 18. An intrahepatic bile duct exhibiting a mild luminal dilatation with eosinophilic secretion, epithelial flattening and a moderate periductal inflammatory cell infiltration. H&E ×100(A) ×400(B).


Table 1
Experimental design

Table 2
Number of worms recovered

Table 3
Number of worms survived

Table 4
Worms recovered from various parts of the body by group

Table 5
Worms recovered from various parts of the body by duration

Table 6
Worm size recovered before and after inoculation by group

Table 7
Worms size recovered before and after inoculation by duration

Table 8
Atrophic changes of reproductive organs of the worms found by group

Table 9
Atrophic changes of reproductive organs of the worms found by duration

Table 10
Histopathological changes of mouse liver

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