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Korean J Parasitol > Volume 15(1):1977 > Article

Original Article
Korean J Parasitol. 1977 Jun;15(1):36-42. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1977.15.1.36
Copyright © 1977 by The Korean Society for Parasitology
Histochemical studies on Echinorhynchus gadi (Acanthocephala)
Jung Kyun Chu,Shin-Yong Kang,Jong-Phil Chu and Dong-Wook Sung
Department of Parasitology, School of Medicine, Kyung Hee University, Korea.

The distribution of glycogen, polysaccharide, mucopolysaccharide, lipid and nucleic acid has been studied in Echinorhynchus gadi(Acanthocephala). The results were summarized as follows.

1. Glycogen and polysaccharide was demonstrated by Bauer PAS reaction technique and was found in fertilization membrane in ovum, central nuclear mass in acanthor and lemnisci, hypodermis in cystacanth.

2. Mucopolysaccharide was demonstrated by Mowry alcian blue staining technique and was found in outer membrane, fibrillar coat, fertilization membrane and inner membrane in acanthocephalan ova.

3. Lipid was demonstrated by Smith Nile blue stain and Lison Sudan black B staining technique and was found roughly parallel to that of polysaccharide.

4. Nucleic acid was demonstrated by Rosenbeck Feulgen reaction, Taft methylgreen-pyronin stain and Diengdoh acridine orange staining technique and found in central nuclear mass in acanthor, also, was found in lemnisci, proboscis and hypodermis in cystacanth.


Explanations of Figures
Fig. 1. Cystacanth stained with PAS reaction (×450).

Fig. 2. Cystacanth stained with Feulgen reaction (×450).

Fig. 3. Acanthor stained with Methyl Green Pyronin (×450).

Fig. 4. Cystacanth stained with Acridine Orange (×450).

Fig. 5. Ova of Acanthocephala stained with Nile Blue (×450).


Table 1
Distribution of carbohydrate, lipid, and nucleic acid in ovum of Echinorhychus gadi

Table 2
Distribution of carbohydrate, lipid, and nucleic acid in cystaxanth of E. gadi

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