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Original Article
Korean J Parasitol. 1976 Dec;14(2):94-102. Korean.
Published online March 20, 1994.  http://dx.doi.org/10.3347/kjp.1976.14.2.94
Copyright © 1976 by The Korean Society for Parasitology
Immunoelectrophoresis for anthelmintics evaluation against experimental paragonimiasis
Won-Young Choi,Kimihiko Kimura and Moriyasu Tsuji
Department of Parasitology, Catholic Medical College, Seoul, Korea.
Department of Parasitology, School of Medicine, Hiroshima University, Hiroshima, Japan.
Abstract

Paragonimiasis is one of the most important endemic diseases in Korea. However, recognition of the limitation of microscopic examination of sputum and feces for Paragonimus eggs has led to the investigation of immunoserological techniques for paragonimiasis.

The other hand, bithionol preparation has been used as a drug of choice in paragonimiasis but recently niclofolan preparation has introduced as a new agent. By the time, the authors attempted the evaluation of above both agents against experimental rat paragonimiasis by immunoelectrophoresis and Ouchterlony test.

The immunoeletrophoresis and Ouchterlony tests were performed according to the method Tsuji and Grabar et Williams respectively, with antigen extracted from lyophilized worm of P. westermani with 0.1 per cemt saline solution. Meanwhile, rats were infected with per os 20 metacercariae of P. westermani above two kinds of serologic tests and were undertaken at biweekly intervals.

Then, bithionol was administered every other day for 5 successively for 2 weeks or 6 weeks after infection. Another groups, niclofolan was administered per os single dose same as above.

1. The sera from the rats infected with P. westermani, after treatment with bithionol or niclofolan preparations began to show the precipitin bands against P. westermani antigen 8 weeks after the infection in immunoelectrophoresis and Ouchterlony test, but from 10 or 12 weeks after infection, the number of bands were decreased or disappeared gradually.

2. In general, the sera from the rats treated with bithionol or niclofolan showed the precipitin bands delaying 2 weeks than control rat groups.

3. The sera from the rats administered with hydrocortisone showed precipitin bands neither in immunoelectrophoresis nor in Ouchterlony test.

Figures


Fig. 1
Precipitin reactions of rat sera infected with P. westermani against the homologous antigens in Ouchterlony test.

Numbers 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27 and 28 are the sera samples from rats bled on VI, VIII, X and XII weeks after infection respectively.



Fig. 2-1
Immunoelectrophoretic patterns of rat sera infected with P. westermani against the homologous antigens.

Numbers 1, 6, 7, 8, 9 and 10 are the sera samples from rats bled on VIII and X weeks after infection respectively.



Fig. 2-2
Numbers 11, 12, 13, 14, 15 and 17 are the sera samples from rats bled on VIII and X weeks after infection respectively.


Fig. 2-3
Numbers 18, 19, 20, 21, 22 and 23 are the sera samples from rats bled on VIII and X weeks after infection respectively.


Fig. 2-4
Numbers 24, 25, 26, 27 and 28 are the sera samples from rats bled on VI, VIII and X weeks after infection respectively.


Fig. 2-5
Numbers 1, 3, 4, 6, 7, 8, 9, 10, 11, 12 and 13 are the sera samples from rats bled on XII weeks after infection respectively.

Tables


Table 1
The number of precipitin bands in Ouchterlony tests between sera from rat infected with metacercaria of P. westermani and the homologous antigens


Table 2
The number of precipitin bands in immunoelectrophoresis between sera from rat infected with metacercaria of P. westermani and the homologous antigens

References
1. Han SH, Woo CK. [Immuno-Diffusion And Complement-Fixation Test In Paragonimiasis Of Cat]. Korean J Parasitol 1969;7(3):160–165.
2. Tada I. Physiological and serological studies of Paragonimus miyazakii infection in rats. J Parasitol 1967;53(2):292–297.
 
3. Yogore MG, et al. Am J Trop Med & Hyg 1965;14:586–591.
4. Yokogawa M, et al. Jpn J Parasit 1961;10(2):302–316.
5. Yokogawa M, et al. Jpn J Parasit 1961;10(2):317–327.
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