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Korean J Parasitol > Volume 13(2):1975 > Article

Original Article
Korean J Parasitol. 1975 Dec;13(2):152-158. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1975.13.2.152
Copyright © 1975 by The Korean Society for Parasitology
Indirect fluorescent antibody test for diagnosis of paragonimiasis
Won-Young Choi,Won-Koo Lee and Ok-Ran Lee
Department of Parasitology, Catholic Medical College, Seoul, Korea.

Indirect fluorescent antibody tests were performed with sera of paragonimiasis patients and skin test positive sera against Paragonimus antigen.

Paragonimus antigen was prepared from lyophilized adult worms of P. westermani by defatting with ethyl-ether before extracting with barbital buffered saline.

Preparation of Paragonimus antigen for the indirect fluorescent antibody test was based upon Sato's method used for sero-diagnosis of anisakiasis, with Sephadex G-25 instead of Sepharose 4B.

The results were as follows:

1. The indirect fluorescent antibody titers of paragonimiasis patient's sera ranged from 1:64 to 1:512, whereas the control sera showed titers of less than 1:16. As controls, Clonorchis patient's sera and parasite-free healthy human sera were used.

2. In indirect fluorescent antibody tests, the skin test positive human sera against Paragonimus antigen showed a positive rate of 41.5 per cent in the case of titers more than 1:40. On the other hand, complement fixation tests on the same sera showed a positive rate of 32.5 per cent in the case of titers more than 1:20.


Fig. 1
Distribution of Immunofluorescent titers and complement fixation titers in Paragonimus skin test positive's sera.


Table 1
Indirect immunofluorescent tests and complement fixation tests in Paragonimus egg detected human sera

Table 2
Distribution of antibody titers in indirect immunofluorescent tests and complement fixation tests in confirmed paragonimiasis

Table 3
Immunofluorescent titers and complement fixation titers in the sera from skin test positives for paragonimiasis

1. Ando A. Chugai Iji Shimpo 1917;900:1122–1130.
2. Ando A. Jpn J Microbiol Soc 1921;15:391–404.
3. Axen R, Porath J, Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature 1967;214(5095):1302–1304.
4. Baratawidjaja RK, et al. Canad J Microb 1963;9:625–628.
5. Chung HL, et al. Chinese Med J 1955;73:368–378.
6. Collins WE, Skinner JC, Contacos PG, Guinn EG. Fluorescent-antibody studies on simian malaria. II. Development of antibodies to Plasmodium cynomolgi. Am J Trop Med Hyg 1967;16(3):267–272.
7. Diggs CL, Sadun EH. Serological Cross Reactivity Between Plasmodium Vivax and Plasmodium Falciparum as Determined by a Modified Fluorescent Antibody Test. Exp Parasitol 1965;16:217–223.
8. Duxbury RE, Sadun EH. Fluorescent Antibody Test for the Serodiagnosis of Visceral Leishmaniasis. Am J Trop Med Hyg 1964;13:525–529.
9. Duxbury RE, Sadun EH. Soluble antigen fluorescent antibody test (SAFA) for human filariasis. Exp Parasitol 1967;20(1):77–82.
10. Fife EH Jr, Muschel LH. Fluorescent-antibody technic for serodiagnosis of Trypanosoma cruzi infection. Proc Soc Exp Biol Med 1959;101(3):540–543.
11. Gore RW, Sadun EH. A soluble antigen fluorescent antibody (SAFA) test for the immunodiagnosis of trichinosis in man and experimental animals. Exp Parasitol 1968;23(3):287–293.
12. Gore RW, Sadun EH, Hoff R. Echinococcus granulosus and E. multilocularis: soluble antigen fluorescent antibody test. Exp Parasitol 1970;28(2):272–279.
13. Kim JS, Park SS. [Values of intradermal test and multiple sputum examinations for Diagnosis of paragonimiasis]. Korean J Parasitol 1974;12(2):119–125.
14. Le Viguelloux J, et al. Trop Dis Bull 1972;69:117–118.
15. Mantovani A, Sulzer AJ. Indirect fluorescent antibody technique for diagnosis of canine filariasis. Am J Vet Res 1967;28(123):351–354.
16. Muller R. Dracunculus medinensis: diagnosis by indirect fluorescent antibody technique. Exp Parasitol 1970;27(3):357–361.
17. Musgrave WE. Philip J Sci 1907;2:15–65.
18. Ritchie LS, et al. J Parasitol 1951;37 Suppl:28–30.
19. Sadun EH, Anderson RI, Williams JS. Fluorescent antibody test for the serological diagnosis of trichinosis. Exp Parasitol 1962;12:423–433.
20. Sadun EH, et al. J Parasitol 1963;49:385–388.
21. Sadun EH, Gore RW. Relative sensitivity and specificity of soluble antigens (metabolic and somatic) and whole cercariae in fluorescent antibody tests for schistosomiasis in humans and rabbits. Exp Parasitol 1967;20(2):131–137.
22. Sato S, et al. Gumma J Med Sci 1964;13:199–205.
23. Seed JR, et al. J Parasitol 1966;52:358–362.
24. Seed JR, et al. Tulane Stud Zool Bot 1968;15:70–74.
25. Sulzer AJ. Indirect fluorescent antibody tests for parasitic diseases. I. Preparation of a stable antigen from larvae of Trichinella spiralis. J Parasitol 1965;51(5):717–721.
26. Sulzer AJ, Hall EC. Indirect fluorescent antibody tests for parasitic diseases. IV. Statistical study of variation in the indirect fluorescent antibody (IFA) test for toxoplasmosis. Am J Epidemiol 1967;86(2):401–407.
27. Tada I. Physiological and serological studies of Paragonimus miyazakii infection in rats. J Parasitol 1967;53(2):292–297.
28. Taffs LF, Voller A. In Vitro Fluorescent Antibody Studies on Ascaris Lumbricoides and Ascaris Suum. Trans R Soc Trop Med Hyg 1963;57:353–358.
29. Toussaint AJ, Anderson RI. Soluble Antigen Fluorescent-Antibody Technique. Appl Microbiol 1965;13:552–558.
30. Toussaint AJ, Tarrant CJ, Anderson RI. An indirect fluorescent antibody technique using soluble antigens for serodiagnosis of Trypanosoma cruzi infection. Proc Soc Exp Biol Med 1965;120(3):783–785.
31. Toussaint AJ. Improvement of the soluble antigen fluorescent-antibody procedure. Exp Parasitol 1966;19(1):71–76.
32. Williams JS, Duxbury RE, Anderson RI, Sadun EH. Fluorescent antibody reactions in Trypanosoma rhodesiense and T. gambiense in experimental animals. J Parasitol 1963;49:380–384.
33. Yogore MG, et al. Amer J Trop Med & Hyg 1965;14:586–591.
34. Zaman V, et al. Trans Roy Soc Trop Med Hyg 1965;59:690–693.
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