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Korean J Parasitol > Volume 13(1):1975 > Article

Original Article
Korean J Parasitol. 1975 Jun;13(1):47-52. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1975.13.1.47
Copyright © 1975 by The Korean Society for Parasitology
Cytochemical study on lipase and nucleic acid in Trichomonas vaginalis
Jung-Kyun Chu and Soe-Bum Chung
Department of Parasitology, School of Medicine, Kyung Hee University, Korea.
Abstract

Cytochemical demonstrations of lipase and nucleic acid in Trichomonas vaginalis were attempted. Modified Gomori tween method for lipase and acridine orange method for nucleic acid was applied. Trichomonas vaginalis incubated in the isolation mediurn (C.P.L.M.) were pooled and fixed using 0.1 N McIlvaine buffer saline and cold acetone for lipase and Walpole acetate buffer saline and acetic alcohol for nucleic acid.

The results were obtained as follows:

Activity of lipase and nucleic acid were recognized in the cytoplasm of T. vaginalis as scattered positive granules stained in blue yellow-green bright reddish color respectively. However these reactions were not shown in nucleus, nuclear membrane, undulating membrane etc. Present authos believe the negative finding of acridine orange staining in nucleus should be studied further.

Figures


Fig. 1
The photograph of the specimen stained with nile blue sulfate for lipase shows blue dots in the cytoplasm represent sites of lipase activity.


Fig. 2
The photograph of the specimen stained with nile blue sulfate for lipase shows blue dots in the cytoplasm represent sites of lipase activity.


Fig. 3
The photograph of the specimen stained with acridine orange for RNA and DNA shows reddish brown granules in the cytoplasm, representing sites of DNA.


Fig. 4
The photograph of the specimen stained with acridine orange for RNA and DNA shows yellowish-green granules in the cytoplasm, representing sites of DNA.


Fig. 5
The photograph of the specimen stained with acridine orange for RNA and DNA shows yellowish-green granules in the cytoplasm, representing sites of DNA.


Fig. 6
The photograph of the specimen stained with acridine orange for RNA and DNA shows yellowish-green granules in the cytoplasm, representing sites of DNA.

References
1. Armstrong JA. Histochemical differentiation of nucleic acids by means of induced fluorescence. Exp Cell Res 1956;11(3):640–643.
  
2. Diengdoh JV. Proc Roy Micr Soc 1966;1:213.
3. Hicks JD, Matthaei E. Fluorescence in histology. J Pathol Bacteriol 1955;70(1):1–12.
  
4. Kunitake G, et al. J Path Bact 1962;70(1):1–12.
5. Muller M, Toth J, Toro I. Increase of esterase activity during intracellular digestion in a histophagous ciliate. Nature 1960;187:65.
  
6. Schummelfeder N. J Histochem Cytochem 1958;6:392–393.
7. Sharma NN, et al. J Histochem Cytochem 1963;11:628–634.
 
8. Suzuoki Z, et al. J Biochem Jpn 1952;39:321–331.
9. Wellerson R Jr, Kupferberg AB. On glycolysis in Trichomonas vaginalis. J Protozool 1962;9:418–424.
 
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