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Korean J Parasitol > Volume 12(2):1974 > Article

Original Article
Korean J Parasitol. 1974 Dec;12(2):101-110. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1974.12.2.101
Copyright © 1974 by The Korean Society for Parasitology
Experimental studies on the second intermediate hosts of Clonorchis sinensis. III. Observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of Clonorchis sinensis in fresh-water fish
Jae Ku Rhee
Department of Veterinary Medicine, College of Agriculture, Jeonbug National University, Korea.
Abstract

In order to know relationship between the clavate cells of epidermis and infectivity of metacercariae of C. sinensis. histological investigation of epidermis was carried out on the 26 species of fresh-water fish collected from the main rivers in Korea.

Results were summarized as it follows:

1. In general clavate cell had a single nucleus at its center. However it was observed in Liobagrus mediadiposalis and Pelteobagrus fuluidraco that clavate cell had two nuclei with and independent so-called light halo of Oxner and in some cases had none of them.

2. In following species; Misgurnus anguillicaudatus, Cobitis taenia, Liobagrus mediadiposalis, Pelteobagrus fuluidraco, Parasilurus asotus and Anguilla japonica, scales were not detected or buried in the dermis layer, and the epidermis was mostly consisted of clavate cells. Among these species, it was observed that no metacercariae were found.

3. In case of Cyprinus carpio, Carassius carassius (crusian carp and golden crusian carp), Sarcocheilichthys czerskii, Pungtungia herzi and Coreoleuciscus splendius, scales were exposed over the epidermis, in which clavate cells were abundantly found. In these species, a few of the metacercariae were detected.

4. On the other hand, no clavate cells were observed in Pseudorasbora parva, Zacco platypus, Microphysogobio koreensis, Gnathopogon majimae, Gnathopogon coreanus, Acheilognathus signifer, Acheilognathus yamatsutae, Cultriculus eigenmanni, Coreoperca herzi, Pseudoperilampus uyekii, Pseudoperilampus notatus and Pseudogobio esocinus. These fishes with exposed scales on the epidermis were well known to serve as the second intermediate hosts of C. sinensis.

From the above results obtained, it was suggested that the existance of clavate cells in the epidermis of the fresh-water fish seems to be correlated with the infectivity of the metacercariae of C. sinensis.

Figures


Figs. 1-4
Figures given are of vertical sections about epidermis of fresh-water fish stained with hematoxylin and eosin.

Fig. 1. Clavate cells and mucous cells are shown, and a clavate cell has so-called light halo of Oxner around nucleus(arrow), Parasilurus asotus, × 1,400

Fig. 2. There are two nuclei with an independent so-called light halo of Oxner(arrow) within a clavate cell, Liobagrus Mediadiposalis, × 1,400

Fig. 3. Clavate cells are visible up to surface of layer of epidermis, Carassius carassius (golden crusian carp), × 1,400

Fig. 4. Only epthelial cells can be observed, Gnathopogon majimae, × 1,800

Abbreviations; C: Clavate cell, Co: Corium, E: Epithelial cell, M: Mucous cell, S: Scale



Figs. 5-14
Figures given are of vertical sections about epidermis of fresh-water fish stained with hematoxylin and eosin.

Fig. 5. Clavate cells of the first layer up to third are shown, Misgurnus anguillicaudatus, × 1,200

Fig. 6. Clavate cells are shown to occupy most area of the median layer of epidermis, Cobitis taenia, × 1,200

Fig. 7. Typical club-shaped clavate cells are observed, Anguilla japonica, × 1,200

Fig. 8. These are clavate cells which locally exist, Carassius carassius(crusian carp), × 1,200

Fig. 9. These are clavate cells which are locally scattered, Sarcocheilichthys czerskii, × 1,200

Fig. 10. These are clavate cells which locally exist, Acanthorhodeus asmussi, × 1,200

Fig. 11. Only epithelial cells and mucous cells can be observed, Pseudorasbora parva, × 1,200

Fig. 12. Only epithelial cells and mucous cells can be observed, Acheilognathus signifer, × 1,200

Fig. 13. Only epithelial cells can be observed, Coreperca herzi, × 1,600

Fig. 14. Only epithelial cells and mucous cells are observed, Ophicephalus argus, × 1,200

Abbreviations; C: Clavate cell, Co: Corium, E: Epithelial cell, M: Mucous cell, S: Scale


Tables


Table 1
Clavate and Mucous Cells at Epidermis in Fresh-water Fish

References
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2. Chun SK. [Studies on the experimental mode of infections of Clonorchis sinensis: III. Studies on the wormicidal effect of external mucous substance of some fresh water fish on the larva of Clonorchis sinensis]. Korean J Parasitol 1964;2(3):148–158.
 
3. Ishida N, et al. Sci Res Hirosaki Univ 1960;7(2):57–63.
4. Kapoor BG. J Zool Soc India 1953;5(2):191–210.
5. Kelly DE. The Leydig cell in larval amphibian epidermis. Fine structure and function. Anat Rec 1966;154(3):685–699.
  
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7. Yamada J. Ann Zool Jpn 1968;41(1):1–8.
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