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Korean J Parasitol > Volume 11(1):1973 > Article

Original Article
Korean J Parasitol. 1973 Apr;11(1):13-20. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1973.11.1.13
Copyright © 1973 by The Korean Society for Parasitology
Autoradiographic study on the uptake of 3H-6-thymidine by Fasciola hepatica
Joong-Ho Kim
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract

Autoradiographic study was performed in order to know the distribution of exogenous radioactive substance, 3H-6-Thymidine, by liver fluke, Fasciola hepatica.

The worms were maintained in vitro for 1 hour, at 37℃, in Tyrode solution containing 3H-6-Thymidine. After the incubation, the worms were sectioned and autoradiographed by applying the techniques of microautoradiography.

The black grains derived from labeled substance were highly accumulated in the vitelline duct and vitelline glands.

Moderate amount of blackening showed in the subcuticular muscle fibers, testes, ovary, cirrus sac, oral sucker and pharynx.

Slightly labeled particles were in the reticular tissue, ventral sucker, intestinal epithelium, prostate, uterus and ova. There can hardly be found the black grains in the cuticle.

Figures


Figs. 1-8
Fig. 1. High concentration of black grains congregated in vitelline glands (×600).

Fig. 2. Normal structure of vitelline glands (×600).

Fig. 3. Spermatogonia at periphery of testis were moderate amount of labeling (×120).

Fig. 4. Normal structure of testis (×120).

Fig. 5. Moderate amount of black grains can be seen in testis at high power magnification (×600).

Fig. 6. High power magnification of normal structure of testis (×600).

Fig. 7. Nuclei of oogonia at periphery of the ovary showed moderate amount of black grains (×120).

Fig. 8. Normal structure of ovary (×120).



Figs. 9-15
Fig. 9. High power magnification of ovary showing high concentration of radioactivity were congregated at periphery (×600).

Fig. 10. Normal structure of ovary (×600).

Fig. 11. Slight amount of black grains were seen at Mehlis' gland (×600).

Fig. 12. Normal structure of Mehlis' gland (×600).

Fig. 13. High power magnification of reticular tissue. Slightly concentrated black grains can be seen in reticulum (×600).

Fig. 14. High power magnification of normal structure of reticular tissue (×600).

Fig. 15. Slightly concentrated black grains in prostste (×600).


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