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Korean J Parasitol > Volume 8(1):1970 > Article

Original Article
Korean J Parasitol. 1970 Apr;8(1):13-21. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1970.8.1.13
Copyright © 1970 by The Korean Society for Parasitology
Immobilization-agglomeration reaction for the diagnosis of Trichomonas vaginalis infection
Pyung Rim Chung
Department of Parasitology, Yonsei University Medical College, Seoul, Korea.

The Trichomonas immobilization-agglomeration reaction was studied using the sera from women with vaginal trichomoniasis and from rabbits inoculated with cultures of T. vaginalis.

It was found that the greatest amount of immobilization occurred at 25 to 30 minutes, and that inactivation of the sera did not affect the results. An evidence of antigenic differences between T. vaginalis, T. hominis and C. albicans was confirmed.

The immobilization reaction was positive in 80.3% of the 71 T. vaginalis positive cases, but in only 7.7% of the 77 persons of T. vaginalis negative cases.

From the above results, it is suggested that immobilization reaction is a useful tool for the diagnosis of T. vaginalis infection.


Fig. 1
Effect of T. vaginalis antiserum against T.vaginalis at its immobilizing and agglomerating titer of 1/16.

……… : Disintegration of cells.

↑ : Complete disintegration.

Fig. 2
Immobility of T. vaginalis as a function of time and the concentration of human antiserum.

— : antiserum, …… : control serum

Fig. 3
Vaginal flagellates which move actively in normal human serum for control of the immobilization-agglomeration test (×400)

Fig. 4
Vaginal flagellates which are immobilized and agglomerated in human antisera (×400)


Table 1
Time and titer for 100% immobilization of T. vaginalis in rabbit antiserum

Table 2
100% immobilization time according to the duration after T.vaginalis inoculation to the rabbit

Table 3
Antigenic relationship between T. vaginalis and T. hominis after treatment with T. vaginalis antiserum for 30 minutes

Table 4
Antigenic relationship between T. vaginalis and C. albicans after treatment with T. vaginalis antiserum

Table 5
Immobility (%) of T. vaginalis as a function of time and concentration of human antiserum

Table 6
Immobilization and agglomeration reaction (I.A.R.) of T. vaginalis in the inactivated or activated antisera

Table 7
Result of I.A.R. for T. vaginalis with human sera

Table 8
Sensitivity and specificity of I.A.R. for diagnosis of Trichomoniasis vaginalis

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