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Korean J Parasitol > Volume 7(1):1969 > Article

Original Article
Korean J Parasitol. 1969 Jun;7(1):48-52. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1969.7.1.48
Copyright © 1969 by The Korean Society for Parasitology
An evaluation of cellophane thick smear technique for mass stool examination
Seung Yull Cho,Soon Hyung Lee,Han Jong Rim and Byong Seol Seo
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.

To evaluate the Kato's cellophane thick smear techcique in detection ability of various helminth ova, authors examined 1,843 nationwidely collected stool specimens by the two methods, formalin-ether technique and cellophane thick smear technique. And the results were analyzed.

1. The positive rates of following helminth ova were superior in cellophane thick smear technique to those of formalin-ether technique: Ascaris lumbricoides(62.3% : 51.1%), Taenia species (1.5% : 0.9%). The difference in A. lumbricoides ova detection is highly significant one. The overall positive rate for helminths ova was also higher in Kato's method (92.3% : 90.0%).

2. The positive fates of following helminth ova were infrior in cellophane thick smear technique to those of formalin-ether technique: Hookworm (5.8% : 14.6%), Trichostrongylus orientalis(3.2% : 17.8%), Clonorchis sinensis(8.3% : 11.0%), Metagonimus yokogawai (0.6% : 1.7%) and Hymenolepis nana(0.05% : 0.3%). The positive rate for the ova of Trichocephalus trichiurus by the two methods was incidentally same, 74.1%.

3. The causes of inferior positive rates in some helminth ova by cellophane thick smear technique were shortly discussed and suggests some opinions in improvement of detection ability.


Table 1
Result of stool examination by formalin-ether technique and cellophane thick smear technique

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