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Korean J Parasitol > Volume 3(1):1965 > Article

Original Article
Korean J Parasitol. 1965 Jun;3(1):39-44. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1965.3.1.39
Copyright © 1965 by The Korean Society for Parasitology
Studies on the intradermal test of Clonorchis peptide(CPT) Antigen 1. Preliminary report
Dong Wik Choi, M.D.,Sang Whon Lee, M.D.,Jung Man Kim, M.D. and Jong Taek Lee, M.D.
Department of Preventive Medicine, School of Medicine, Kyungpook National University, Korea.

Polypeptide antigen as isolated from adult worms of Clonorchis sinensis by means of diluted HCl-extraction method. Preliminary intradermal examination with those polypeptide antigen(CPT) were performed on patients of clonorchiasis, paragonimiasis and metagonimiasis and the result of the intradermal test with CPT antigen were compared with those of Clonorchis protein fraction(CPF) and Veronal buffer saline extraction (VBS) antigen.

The result of the examination were summerized as follows.

1. When 0.05 ml (1 microgram) of CPT was injected intradermally, the positive intradermal reaction appeared in 42.7 per cent (37 of 82) on the patients of clonorchiasis.

2. The sizes of the wheal and erythema with CPT antigen was smaller than that of CPF and VBS antigen.

3. Intradermal reaction with CPT antigen was carried out for other parasitic diseases, such as paragonimiasis and metagonimiasis. The result indicated that CPT antigen may be showed no cross reactions.

4. Clonorchiasis pateints who with negative reaction to CPT antigen, reacted negatively to CPF and VBS antigens.


Fig. 1
Frequency Distribution of Enlarged wheal sizes in 82 Clonorchiasis patients.

Fig. 2
Comparsion of the increased diameters of the wheals between CPE and CPT antigens.

Fig. 3
Comparsion of the increased diameters of the wheals between VBS and CPT antigens.

Fig. 4
Comparsion of the increased diameters of the wheals between VBS and CPF antigens.


Table 1
Isolation of Peptide Antigen.

Table 2
Results of qualitative analysis with CPT.

Table 3
Cutaneous reaction to a C. sinensis Peptide Antigen (CPT) in person with clonorchiasis, paragonimiasis.Metagonimiasis and in non-infected controls.

Table 4
Comparison of results of intradermal test with CPT, CPF and VBS(K) antigens in 82 Clonorchiasis patients.

8. Chung HL, Weng HC, Hou TC, Ho LY. Cross intradermal reactions of patients with paragonimiasis, clonorchiasis and schistosomiasis to different trematode antigens and their clinical significance. Chin Med J 1955 Sep-Oct;73(5):368–378.
13. Okada Y, Morisawa S, Syojima K, Kitagawa M, Nakashima S, Yamamura Y. Improved Method for the Isolation and Properties of Tuberculin Active Peptides. J Biochem 1963;54:484–490.
14. Sadun EH, Walton BC, Buck AA, Lee BK. The use of purified antigens in the diagnosis of clonorchiasis sinensis by means of intradermal and complement fixation tests. J Parasitol 1959 Apr;45(2):129–134.
18. Wykoff DE. Studies on Clonorchis sinensis. II. Development of an antigen for complement fixation and studies on the antibody response in infected rabbits. Exp Parasitol 1959 Feb;8(1):51–57.
20. Morisawa S, Tanaka A, Shojima K, Yamamura Y. Studies on tuberculin active peptide. I. The isolation, crystallization and properties of tuberculin active peptide from tubercle bacillus. Biochim Biophys Acta 1960;38:252–258.
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