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Korean J Parasitol > Volume 2(1):1964 > Article

Original Article
Korean J Parasitol. 1964 Jun;2(1):91-96. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1964.2.1.91
Copyright © 1964 by The Korean Society for Parasitology
Paper chromatographic study on the amino acids of some parasitic helminths
Soon Hyong Lee,Byong Seol Seo and Joo Soo Yoon
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.

1) Unidimensional and two dimensional paper choromatogram were prepared of 10 kinds of parastic helminths.

2) Fourteen amino acids were identified from the acid hydrolysed tissue proteins of A. lumbricoides(cuticle and musculature), A. galli, F. hepatica, E. pancreaticum, P. cervi, T. solium, and M. expansa. They were glycine, alanine, serine, threonine, methione, valine, leucine, aspartic acid, lysine, arginine, tyrosine, proline and histidine. In hydrolysates of A. lumbricoides(female genital organ) and C. sinensis, 13 amino acids were recovered. Twelve amino acid from A. lumbricoides(intestinal tract), 9 from P. westermani, and 6 from H. nana were also identified in the tissue hydrolysates.


Fig. 1
Position of 14 amino acids identified from the acid hydrolysed tissue protein of F. hepatica after two dimensional chromatography.

Asp: Aspartic acid, Arg: Arginine, Ala: Alanine, Glu: Glutamic acid, Gly: Glycine, His: Histidine, Leu: Leucine, Lys: Lysine, Met: Methionine, Pro: Proline, Ser: Serine, Thr: Threonine, Tyr: Tyrosine, Val: Valine.


Table 1
Amino acids isolated from various worms.

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