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Korean J Parasitol > Volume 2(1):1964 > Article

Original Article
Korean J Parasitol. 1964 Jun;2(1):41-51. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1964.2.1.41
Copyright © 1964 by The Korean Society for Parasitology
Studies on Microphalloides japonicus
Byong Seol Seo, Joo Soo Yoon, Chan Wuk Lee and Soon Hyung Lee
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.

Metacercariae of Microphalloides japonicus were isolated from five kinds of crab which were collected at the Delta areas of Naktong river. These metacercariae were fed to experimental animals. Some of these metacercariae were also incubated in screw cap tube at 37℃. The longevity and developmental difference between flukes grown in vivo and in vitro were described.

1) The total number of crabs examined were 2245 and Helice tridens tridens showed highest incidence among five species of brackish water crabs.

2) Some of these metacercariae were fed experimentally to mice and rats, and the mature worms were obtained in the host intestine 24, 48, and 72 hours after infection. The adult and larval morphology of this fluke was described.

3) Utilizing three kinds of medium, the adult worms of Microphalloides japonicus have been successfully developed under axenic conditions in vitro. Eggs containing adult worm were obtained in all of three media, i.e., Ringer's solution, Tyrode's solution, and Serum-Tyrode (1:1) solution. The adult worms were morphologically similar to flukes grown in vivo, but there were certain degree of difference between adult flukes grown in vivo and in vitro.


Fig. 1
Geographical illustration of brackish area where crabs were collected.

Fig. 2
Metacercariae in crab tissue.

Fig. 3
Metacercariae in low power magnification.

Fig. 4
Metacercaria. Note V or Y shaped excretory bladder.

Fig. 5
Excysted metacercaria. Note already matured reproductive organs.

Fig. 6
Adult fluke recovered from rat host which was killed 24 hours after infection. Some eggs are observable in the uterine tubule.

Fig. 7
Adult fluke recovered from albino rat, 48 hours after infection. Numerous eggs are found in the posterior half of the worm body.

Fig. 8
Adult fluke maintained for 24 hours in vitro (Tyrode's solution). Arrow indicates the egg which appeared for the first time.

Fig. 9
Adult fluke maintained for 48 hours in Tyrode's solution. Note the disfigured vitelline follicles and testes.

Fig. 10
Adult fluke maintained for 5 days in Serum-Tyrode(1:1) medium.

Fig. 11
Adult fluke maintained for 10 days in Tyrode's solution. Testes are disppeared, and the ovary is scarcely observable.


Table 1
Measurement of encysted metacercaria.

Each figure represents the mean value abtained from 20 metacercariae.

Table 2
Measurements of 20 excysted metacercariae.

Table 3
Measurement of adult Microphalloides japonicus recovered from albino rat.

Table 4
Comparative survival of Microphalloides japonicus in virto.

Table 5
Measurements of 20 adult Microphalloides japonicus maintained in vitro (Semichon's acetocarmine stained specimen).

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