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Korean J Parasitol > Volume 36(1):1998 > Article

Original Article
Korean J Parasitol. 1998 Mar;36(1):47-53. English.
Published online Mar 20, 1998.  http://dx.doi.org/10.3347/kjp.1998.36.1.47
Copyright © 1998 by The Korean Society for Parasitology
Identical small subunit ribosomal RNA gene nucleotide sequence of bovine Theileria isolates (Korea and Japan) and Theileria buffeli (Marula, Kenya)
J S Chae,1O D Kwon,2P J Holman,1S D Waghela,1G G Wagner,1 and J M Lee*2
1Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A & M University, College Station, TX, USA.
Received September 22, 1997; Accepted December 02, 1997.


Small subunit ribosomal RNA (SSU rRNA) gene nucleotide sequences of bovine Theileria isolates from Korea (KLS and KCB) and Japan (JHS) were determined. The genes from each isolate were amplified by the polymerase chain reaction and the approximately 1.8 kb product cloned and sequenced by a modified dideoxynucleotide method. Overlapping gene segments produced with a series of primers were sequenced, resulting in a complete DNA sequence for both forward and reverse strands of the SSU rRNA genes of each isolate. SSU rRNA gene sequences (termed Type A) were identical among the bovine Theileria isolates from Korea and the isolate from Japan. A GenBank data library homology search showed the sequence to be the same as that listed as Theileria buffeli isolated from cattle in Marula, Kenya.


Fig. 1
Agarose gel stained with 2% ethidium bromide of amplified small subunit ribosomal RNA (18S rRNA) genes from a bovine Theileria laboratory stock from Changsu-gun Chonbuk, Korea (KLS), a bovine Theileria sp. from Kimje-gun Chonbuk, Korea (KCB), and a bovine Theileria sp. from Shintoku in Hokkaido, Japan (JHS). The first lane contains a 1 kb DNA ladder marker (Gibco-BRL).

Fig. 2
Sequence alignment of the Type A small subunit ribosomal RNA gene sequence (GenBank Accession) No. U97047) from the Korean (KLS and KCB) and Japanese (JHS) Theileria isolates with the corresponding gene sequence from Theileria buffeli from GenBank database (GenBank Accession No. Z15106).

1. Allsopp BA, Allsopp MT. Theileria parva: genomic DNA studies reveal intra-specific sequence diversity. Mol Biochem Parasitol 1988;28(1):77–83.
2. Allsopp BA, Baylis HA, Allsopp MT, Cavalier-Smith T, Bishop RP, Carrington DM, Sohanpal B, Spooner P. Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences. Parasitology 1993;107(Pt 2):157–165.
3. Allsopp B, Carrington M, Baylis H, Sohal S, Dolan T, Iams K. Improved characterization of Theileria parva isolates using the polymerase chain reaction and oligonucleotide probes. Mol Biochem Parasitol 1989;35(2):137–147.
4. Allsopp MT, Cavalier-Smith T, De Waal DT, Allsopp BA. Phylogeny and evolution of the piroplasms. Parasitology 1994;108(Pt 2):147–152.
5. Chae JS, et al. Korean J Vet Res 1994;34:387–394.
6. Chae JS, et al. Korean J Vet Res 1996;36:187–193.
8. Ellis J, Hefford C, Baverstock PR, Dalrymple BP, Johnson AM. Ribosomal DNA sequence comparison of Babesia and Theileria. Mol Biochem Parasitol 1992;54(1):87–95.
9. Elwood HJ, Olsen GJ, Sogin ML. The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata. Mol Biol Evol 1985;2(5):399–410.
10. Gajadhar AA, Marquardt WC, Hall R, Gunderson J, Ariztia-Carmona EV, Sogin ML. Ribosomal RNA sequences of Sarcocystis muris, Theileria annulata and Crypthecodinium cohnii reveal evolutionary relationships among apicomplexans, dinoflagellates, and ciliates. Mol Biochem Parasitol 1991;45(1):147–154.
12. Kawazu S, Kamio T, Sekizaki T, Fujisaki K. Theileria sergenti and T. buffeli: polymerase chain reaction-based marker system for differentiating the parasite species from infected cattle blood and infected tick salivary gland. Exp Parasitol 1995;81(4):430–435.
13. Kawazu S, Sugimoto C, Kamio T, Fujisaki K. Antigenic differences between Japanese Theileria sergenti and other benign Theileria species of cattle from Australia (T. buffeli) and Britain (T. orientalis). Parasitol Res 1992;78(2):130–135.
14. Kawazu S, Sugimoto C, Kamio T, Fujisaki K. Molecular cloning and immunological analysis of immunodominant piroplasm surface proteins of Theileria sergenti and T. buffeli. J Vet Med Sci 1992;54(2):305–311.
15. Kawazu S, Sugimoto C, Kamio T, Fujisaki K. Analysis of the genes encoding immunodominant piroplasm surface proteins of Theileria sergenti and Theileria buffeli by nucleotide sequencing and polymerase chain reaction. Mol Biochem Parasitol 1992;56(1):169–175.
18. Ngumi PN, Lesan AC, Williamson SM, Awich JR, Morzaria SP, Dolan TT, Shaw MK, Young AS. Isolation and preliminary characterisation of a previously unidentified Theileria parasite of cattle in Kenya. Res Vet Sci 1994;57(1):1–9.
19. Onuma M. Workshop summary: haemospordia. Vet Parasitol 1996;64(1-2):167–169.
20. Pace NR, Olsen GJ, Woese CR. Ribosomal RNA phylogeny and the primary lines of evolutionary descent. Cell 1986;45(3):325–326.
23. Sugimoto C, Sato M, Kawazu S, Kamio T, Fujisaki K. Purification of merozoites of Theileria sergenti from infected bovine erythrocytes. Parasitol Res 1991;77(2):129–131.
24. Uilenberg G, Perie NM, Spanjer AA, Franssen FF. Theileria orientalis, a cosmopolitan blood parasite of cattle: demonstration of the schizont stage. Res Vet Sci 1985;38(3):352–360.
25. Wilson AC, Carlson SS, White TJ. Biochemical evolution. Annu Rev Biochem 1977;46:573–639.
26. Yakimoff WL, et al. Arch Protistenkd 1930;72:176–189.
27. Yakimoff WL, et al. Arch Protistenkd 1931;75:179–190.
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