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Korean J Parasitol > Volume 34(3):1996 > Article

Brief Communication
Korean J Parasitol. 1996 Sep;34(3):211-213. English.
Published online Sep 20, 1996.  http://dx.doi.org/10.3347/kjp.1996.34.3.211
Copyright © 1996 by The Korean Society for Parasitology
Electrophoretic variations of enzyme, GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4) in characterizing clones and isolates of Malaysian Plasmodium falciparum
H H Ang,*1K L Chan,1 and J W Mak2
1School of Pharmaceutical Sciences, University of Science Malaysia, Minden, 11800, Filaria, Malaysia.
Received December 09, 1995; Accepted August 26, 1996.

Abstract

Malaysian, African and Thai Plasmodium falciparum isolates were cultured in vitro by the Trager and Jensen method (1976; 1977) and were later cloned by the limiting dilution method (Rosario, 1981). Forty- eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.

Tables


Table 1
GDH isoenzyme variants for Plasmodium falciparum isolates and clones

References
1. Ang HH, et al. Jpn J Parasitol 1995;45(5):377–382.
3. Rosario V. Cloning of naturally occurring mixed infections of malaria parasites. Science 1981;212(4498):1037–1038.
  
4. Roth EF Jr, Raventos-Suarez C, Perkins M, Nagel RL. Glutathione stability and oxidative stress in P. falciparum infection in vitro: responses of normal and G6PD deficient cells. Biochem Biophys Res Commun 1982;109(2):355–362.
  
5. Sanderson A, Walliker D, Molez JF. Enzyme typing of Plasmodium falciparum from African and some other Old World countries. Trans R Soc Trop Med Hyg 1981;75(2):263–267.
  
6. Sherman IW. Transport of amino acids and nucleic acid precursors in malarial parasites. Bull World Health Organ 1977a;55(2-3):211–225.
7. Sherman IW. Amino acid metabolism and protein synthesis in malarial parasites. Bull World Health Organ 1977b;55(2-3):265–276.
9. Thaithong S, Sueblinwong T, Beale GH. Enzyme typing of some isolates of Plasmodium falciparum from Thailand. Trans R Soc Trop Med Hyg 1981;75(2):268–270.
  
11. Trager W, et al. Bull WHO 1977;5:363–365.
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