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Korean J Parasitol > Volume 33(4):1995 > Article

Brief Communication
Korean J Parasitol. 1995 Dec;33(4):391-394. English.
Published online Dec 20, 1995.  http://dx.doi.org/10.3347/kjp.1995.33.4.391
Copyright © 1995 by The Korean Society for Parasitology
Comparison of acridine orange and giemsa stains for malaria diagnosis
Hyun-Hee Kong and Dong-Il Chung*
Department of Parasitology, Kyungpook National University School of Medicine, Taegu 700-422, Korea.
Received September 12, 1995; Accepted October 20, 1995.


Recently, the Acridine orange (AO) staining method has improved for identification of malaria parasites. Fixed and preserved blood smears of malaria patients were used for comparative analysis of AO and Giemsa stains. The AO staining method required less time and was more sensitive under lower magnification than the Giemsa staining method. The AO staining method provides an alternative to Giemsa for malaria diagnosis in the field and laboratory.


Fig. 1
Intraerythrocytic malaria parasites. A. applique form, (a) Giemsa stain, (b) AO; B. double infection, AO; C. early trophpzoite (ring form), (a) Giemsa stain, (b) AO; D. late trophozioite, AO; E. double dots, (a) Giemsa stain, (b) AO; F. trophozoites in reticulocyte, AO; G.P. vivax gametocyte, Giemsa stain; H. schizont, (a) Giemsa stain, (b) AO; Bars indicate 10 µm.

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