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Korean J Parasitol > Volume 33(1):1995 > Article

Original Article
Korean J Parasitol. 1995 Mar;33(1):55-60. English.
Published online Mar 20, 1995.  http://dx.doi.org/10.3347/kjp.1995.33.1.55
Copyright © 1995 by The Korean Society for Parasitology
IgA response in mice infected with Neodiplostomum seoulensis
Sun Huh,*1Soo-Ung Lee,1Moo-Ho Won,2Young-Gil Jeong,2Young-Hyun Kwon,3 and Chang Sig Choi3
1Department of Parasitology, College of Medicine, Hallym University, Chunchon 200-702, Korea.
2Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, Korea.
3Department of General Surgery, College of Medicine, Hallym University, Chunchon 200-702, Korea.
Received December 31, 1994; Accepted February 25, 1995.

Abstract

To observe the production of IgA in Balb/c mice with neodiplostomiasis, 20 mice were infected with each 200 metacercariae of Neodiplostomum seoulenis. Sera and the duodenums were obtained 3, 7, 14, 28 days post-infection (PI) from five mice each group. Neodiplostomum specific IgA in serum by the enzyme-linked immunosorbent assay increased from 7 days PI and persisted till 28 days PI. Immunohistochemistry for IgA was done with sections of the duodenum. The IgA-positive reaction was generally seen in the lamina propria and submucosa. Some of epithelial cells were positive at 7 and 14 days PI. The present finding showed that Neodiplostomun specific IgA antibody increased in serum and that there was local reaction of IgA in the mucosa and submucosa of the duodenum but not directly related with worm expulsion.

Figures


Fig. 1
Neodiplostomum seoulensis specific IgA in mice sera ater infection. At 28 days PI, the absorbance was significantly increased in comparison to that of control (t-test, p < 0.05).


Fig. 2
Mouse duodenum stained immnuohistochemically for the non-specific IgA antibody. Fig. 2A. Mouse duodenum, control. The villi to crypt ratio was 3:1-4:1. IgA positive reaction (brown color) is shown in the lamina propria and submucosa, ×100. Fig. 2B.ibid. Asterisk (*) in 2A, ×400. Fig. 2C. mouse duodenum 7 days PI. Villi to crypt ratio is about 1:1. IgA positive reaction was shown more in the lamina propria of the villi than in area between crypts. Arrow heads shows the stained epithelial cells, ×1000. Fig. 2D.ibid. Asterisk (*) in 2C, ×400. Fig. 2E. Mouse intestine 28 days PI. Villi to crypt ratio is 1:1 to 2:1. Weak IgA-positive reaction is shown in the lamina propria and submucosa, ×100. Fig. 2F.ibid. Asterisk (*) in 2E, ×400.

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