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Korean J Parasitol > Volume 33(1):1995 > Article

Original Article
Korean J Parasitol. 1995 Mar;33(1):55-60. English.
Published online Mar 20, 1995.  http://dx.doi.org/10.3347/kjp.1995.33.1.55
Copyright © 1995 by The Korean Society for Parasitology
IgA response in mice infected with Neodiplostomum seoulensis
Sun Huh,*1Soo-Ung Lee,1Moo-Ho Won,2Young-Gil Jeong,2Young-Hyun Kwon,3 and Chang Sig Choi3
1Department of Parasitology, College of Medicine, Hallym University, Chunchon 200-702, Korea.
2Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, Korea.
3Department of General Surgery, College of Medicine, Hallym University, Chunchon 200-702, Korea.
Received December 31, 1994; Accepted February 25, 1995.


To observe the production of IgA in Balb/c mice with neodiplostomiasis, 20 mice were infected with each 200 metacercariae of Neodiplostomum seoulenis. Sera and the duodenums were obtained 3, 7, 14, 28 days post-infection (PI) from five mice each group. Neodiplostomum specific IgA in serum by the enzyme-linked immunosorbent assay increased from 7 days PI and persisted till 28 days PI. Immunohistochemistry for IgA was done with sections of the duodenum. The IgA-positive reaction was generally seen in the lamina propria and submucosa. Some of epithelial cells were positive at 7 and 14 days PI. The present finding showed that Neodiplostomun specific IgA antibody increased in serum and that there was local reaction of IgA in the mucosa and submucosa of the duodenum but not directly related with worm expulsion.


Fig. 1
Neodiplostomum seoulensis specific IgA in mice sera ater infection. At 28 days PI, the absorbance was significantly increased in comparison to that of control (t-test, p < 0.05).

Fig. 2
Mouse duodenum stained immnuohistochemically for the non-specific IgA antibody. Fig. 2A. Mouse duodenum, control. The villi to crypt ratio was 3:1-4:1. IgA positive reaction (brown color) is shown in the lamina propria and submucosa, ×100. Fig. 2B.ibid. Asterisk (*) in 2A, ×400. Fig. 2C. mouse duodenum 7 days PI. Villi to crypt ratio is about 1:1. IgA positive reaction was shown more in the lamina propria of the villi than in area between crypts. Arrow heads shows the stained epithelial cells, ×1000. Fig. 2D.ibid. Asterisk (*) in 2C, ×400. Fig. 2E. Mouse intestine 28 days PI. Villi to crypt ratio is 1:1 to 2:1. Weak IgA-positive reaction is shown in the lamina propria and submucosa, ×100. Fig. 2F.ibid. Asterisk (*) in 2E, ×400.

1. Agger MK, Simonsen PE, Vennervald BJ. The antibody response in serum, intestinal wall and intestinal lumen of NMRI mice infected with Echinostoma caproni. J Helminthol 1993;67(3):169–178.
2. Heyworth MF. Intestinal IgA responses to Giardia muris in mice depleted of helper T lymphocytes and in immunocompetent mice. J Parasitol 1989;75(2):246–251.
3. Hong ST. Studies On Intestinal Trematodes In Korea: VII. Growth, Development And Recovery Of Fibricola Seoulensis From Experimentally Infected Rats And Mice. Korean J Parasitol 1982;20(2):112–121.
4. Hong ST, Chai JY, Lee SH. Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus. Korean J Parasitol 1986;24(1):95–97.
5. Hong ST, Cho TK, Hong SJ, Chai JY, Lee SH, Seo BS. Fifteen human cases of Fibricola seoulensis infection in Korea. Korean J Parasitol 1984;22(1):61–65.
6. Hong ST, Shoop WL. Neodiplostomum seoulensis n. comb. (Trematoda: Neodiplostomidae). J Parasitol 1994;80(4):660–663.
7. Huh S, Lee SU, Huh SC. A follow-up examination of intestinal parasitic infections of the Army soldiers in Whachon-gun, Korea. Korean J Parasitol 1994;32(1):61–63.
8. Kho WG, et al. Seoul J Med 1990;31:191–199.
11. Lee SH, Yoo BH, Hong ST, Chai JY, Seo BS, Chi JG. A histopathological study on the intestine of mice and rats experimentally infected by Fibricola Seoulensis. Korean J Parasitol 1985;23(1):58–72.
12. Leyva O, Rico G, Ramos F, Moran P, Melendro EI, Ximenez C. Inhibition of adhesion process mediated by anti-Entamoeba histolytica specific monoclonal IgA antibodies. Arch Med Res 1992;23(2):227–229.
13. Nash PV, Speer CA. B-lymphocyte responses in the large intestine and mesenteric lymph nodes of mice infected with Eimeria falciformis (Apicomplexa). J Parasitol 1988;74(1):144–152.
14. Choi DW, Ock MS, Suh JW. Recent Demonstration Of Helminth Eggs And Larvae From Vegetable Cultivating Soil. Korean J Parasitol 1982;20(2):83–92.
15. Van Loveren H, Osterhaus AD, Nagel J, Schuurman HJ, Vos JG. Detection of IgA antibodies and quantification of IgA antibody-producing cells specific for ovalbumin or Trichinella spiralis in the rat. Scand J Immunol 1988;28(3):377–381.
16. Vinayak VK, Khanna R, Kum K. Kinetics of intraepithelium and lamina propria lymphocyte responses during Giardia lamblia infection in mice. Microb Pathog 1991;10(5):343–350.
17. Yu JR, Hong ST, Chai JY, Lee SH. The effect of reinfection with Neodiplostomum seoulensis on the histopathology and activities of brush border membrane bound enzymes in the rat small intestine. Korean J Parasitol 1995;33(1):37–43.
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