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Korean J Parasitol > Volume 32(1):1994 > Article

Original Article
Korean J Parasitol. 1994 Mar;32(1):13-18. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1994.32.1.13
Copyright © 1994 by The Korean Society for Parasitology
Promoting role of Clonorchis sinensis infection on induction of cholangiocarcinoma during two-step carcinogenesis
J H Lee,*H M Yang,U B Bak and H J Rim
Department of Parasitology and Institute for Tropical Endemic Diseases, College of Medicine, Korea University, Seoul 136-705, Korea.
Received January 29, 1994; Accepted March 04, 1994.


Chronic Clonorchis sinensis (CS) infection is etiologically related to cholangiocarcinoma (CHCA) in human and animals. This study was carried out to clarify the role of CS infection on dimethylnitrosamine (DMN)-induced cholangiocarcinogenesis. Fifteen hamsters were administered with 15 ppm DMN for 4 weeks and one week later, the hamsters were infected with 15 metacercariae of CS (DMN→CS group). The other 15 hamsters were infected with CS and after 5 weeks they were treated with the drug, praziquantel. Again one week later, the hamsters were administered with DMN (CS→DMN group). The other 15 hamsters were administered with DMN and CS simultaneously (CS+DMN group). Histopathological examination of the livers showed CHCA with papillary or adenomatous hyperplasia of bile ductules in 3 of 15 hamsters of DMN→CS group and in 11 of 15 hamsters of DMN+CS group. These results suggest that CS infection to hamsters may have a promoting effect on the development of CHCA.


Fig. 1
Experimental design for study of the role of C. sinensis infection on the development of cholangiocarcinoma.


Table 1
Gross findings of the livers in the hamsters in each group

Table 2
Histopathological changes and tumor incidence in the livers of the hamsters of each group

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