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Korean J Parasitol > Volume 31(2):1993 > Article

Original Article
Korean J Parasitol. 1993 Jun;31(2):149-156. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.2.149
Copyright © 1993 by The Korean Society for Parasitology
Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies
T S Yong,*1I S Yeo,1J H Seo,1J K Chang,1J S Lee,2T S Kim,2 and G H Jeong3
1Department of Parasitology, Yonsei University College of Medicine, Seoul 120-752, Korea.
Received February 26, 1993; Accepted March 29, 1993.


Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas secreting species-specific anticysticercal Mabs (Cya-1, Cya-7, Cya-28 and Cya-31) were selected. Each Mab reacted on antigenic components of 25.5 kDa (Cya-1), 28 kDa (Cya-7), 87.5 kDa (Cya-28), and 12.5 kDa (Cya-31). IFA showed that Cya-1 was located at the calcium corpuscles, and Cya-7 at the loose connective tissue of T. solium metacestode scolex. Cya-28 and Cya-31 reacted on the tegument of the scolex. By conventional ELISA, 23 out of 28 (82.1%) cysticercosis patients were found serologically positive, but 1 out of 9 (11.1%) sparganosis cases and 6 out of 31 (19.4%) paragonimiasis cases showed false positives. By ELISA-inhibition test using species-specific anti-cysticercal Mab Cya-7, 19 out of 28 (67.9%) cysticercosis cases were found serologically positive, but there were no false positives in other parasitic infections.


Fig. 1
SDS-PAGE pattern of crude scolex extract of T. solium metacestodes in 5-15% linear gradient gel, stained gel, stained with Coomassie brilliant blue R-250.

Fig. 2
Findings of immunoblots reacted on species-specific anti-cysticercal Mabs: 1) Cya-1 (25.5 kDa), 2) Cya-7 (28 kDa), 3) Cya-28 (87.5 kDa), 4) Cya-31 (12.5 kDa), 5) immune mouse serum and 6) the protein band pattern of crude scolex extract of T. solium metacestodes on a nitrocellulose paper stained with Amido-Black B.

Fig. 3
Localization of Mabs by indirect fluorescent antibody technique. Frozen sections of scolices of T. solium metacestode were reacted on: (A) Cya-1 showing positive reactions at calcium corpuscles (×100), (B) a magnification (×200) of Fig. 1, (C) Cya-7 showing positive reactions at the loose connective tissue of a scolex (×100), (D) Cya-31 showing positive reactions at the tegument of the parasite (×100), and (E) immune mouse serum as a positive control. (▾: calcium corpuscle, C: loose connective tissue, T: tegument).

Fig. 4
Distribution of absorbance values of human sera in cysticercosis (Cy), sparganosis (Sp), paragonimiasis (Pw) cases and normal controls (NHS) against crude scolex extract of T. solium metacestodes by conventional ELISA. Twenty-three out of 28 (82.1%) cysticercosis patients were found pasitive, but 1 out of 9 (11.1%) sparganosis cases and 6 out of 31 (19.4%) paragonimiasis cases showed false positive.

Fig. 5
Inhibition rate (%) of Mab by human sera in the ELISA-inhibition test using species-specific anti-cysticercal Mab (Cya-7) directed against an exposing antigen in infection for serodiagnosis of cysticercosis. The cut-off value is set at 12%. Nineteen out of 28 (67.9%) cysticercosis cases were found positive, and there were no false positives in other infections.

1. Arambulo PV 3rd, Walls KW, Bullock S, Kagan IG. Serodiagnosis of human cysticercosis by microplate enzyme-linked immunospecific assay (ELISA). Acta Trop 1978;35(1):63–67.
2. Cabrera Z, Parkhouse RM, Forsyth K, Gomez Priego A, Pabon R, Yarzabal L. Specific detection of human antibodies to Onchocerca volvulus. Trop Med Parasitol 1989;40(4):454–459.
3. Cho SY, Kang SY, Kim SI. Analysis of antigen specificity using monoclonal and polyclonal antibodies to Cysticercus cellulosae by enzyme-linked immunoelectrotransfer blot technique. Korean J Parasitol 1987;25(2):159–167.
4. Cho SY, Kim SI, Kang SY, Choi DY, Suk JS, Choi KS, Ha YS, Chung CS, Myung HJ. Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean J Parasitol 1986;24(1):25–41.
5. Cho SY, Kim SI, Kang SY, Kong Y. Biochemical properties of a purified protein in cystic fluid of Taenia solium metacestodes. Korean J Parasitol 1988;26(2):87–94.
6. Cho SY, Kong Y, Kim SI, Kang SY. Measurement of 150 kDa protein of Taenia solium metacestodes by antibody-sandwich ELISA in cerebrospinal fluid of neurocysticercosis patients. Korean J Parasitol 1992;30(4):299–307.
7. Coker-Vann M, Brown P, Gajdusek DC. Serodiagnosis of human cysticercosis using a chromatofocused antigenic preparation of Taenia solium cysticerci in an enzyme-linked immunosorbent assay (ELISA). Trans R Soc Trop Med Hyg 1984;78(4):492–496.
8. Di Felice G, Pini C, Afferni C, Vicari G. Purification and partial characterization of the major antigen of Echinococcus granulosus (antigen 5) with monoclonal antibodies. Mol Biochem Parasitol 1986;20(2):133–142.
9. Diaz JF, Verastegui M, Gilman RH, Tsang VC, Pilcher JB, Gallo C, Garcia HH, Torres P, Montenegro T, Miranda E. Immunodiagnosis of human cysticercosis (Taenia solium): a field comparison of an antibody-enzyme-linked immunosorbent assay (ELISA), an antigen-ELISA, and an enzyme-linked immunoelectrotransfer blot (EITB) assay in Peru. The Cysticercosis Working Group in Peru (CWG). Am J Trop Med Hyg 1992;46(5):610–615.
10. Eom KS, Cho SY, Rim HJ. Comparative evaluation of indirect immunofluorescent antibody test with enzyme-linked immunosorbent assay in serodiagnosis of human neurocysticercosis. Korean J Parasitol 1988;26(1):27–32.
11. Espinoza B, Ruiz-Palacios G, Tovar A, Sandoval MA, Plancarte A, Flisser A. Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis. J Clin Microbiol 1986;24(4):536–541.
12. Gottstein B, Zini D, Schantz PM. Species-specific immunodiagnosis of Taenia solium cysticercosis by ELISA and immunoblotting. Trop Med Parasitol 1987;38(4):299–303.
13. Jaffe CL, McMahon-Pratt D. Serodiagnostic assay for visceral leishmaniasis employing monoclonal antibodies. Trans R Soc Trop Med Hyg 1987;81(4):587–594.
14. Kim CH, Yang J. Imunological characterization of antigens from cysticercus and sparganum and their application to immunodiagnosis 1. Immunological characteristics of crude antigenic components from Cysticercus cellulosae. Korean J Parasitol 1988;26(4):245–254.
15. Kim SI, Kang SY, Cho SY, Hwang ES, Cha CY. Purification of cystic fluid antigen of Taenia solium metacestodes by affinity chromatography using monoclonal antibody and its antigenic characterization. Korean J Parasitol 1986;24(2):145–148.
16. Knobloch J, Delgado E. Immunodiagnosis of cysticercosis: standardization of ELISA and its application to field conditions. Trop Med Parasitol 1985;36(3):157–159.
17. Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975;256(5517):495–497.
18. Kong Y, Kang SY, Cho SY, Min DY. Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium. Korean J Parasitol 1989;27(2):131–139.
19. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680–685.
20. Larralde C, Montoya RM, Sciutto E, Diaz ML, Govezensky T, Coltorti E. Deciphering western blots of tapeworm antigens (Taenia solium, Echinococcus granulosus, and Taenia crassiceps) reacting with sera from neurocysticercosis and hydatid disease patients. Am J Trop Med Hyg 1989;40(3):282–290.
21. Liu D, Lightowlers MW, Rickard MD. Evaluation of a monoclonal antibody-based competition ELISA for the diagnosis of human hydatidosis. Parasitology 1992;104(Pt 2):357–361.
22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
23. Mitchell GF, Premier RR, Garcia EG, Hurrell JG, Chandler HM, Cruise KM, Tapales FP, Tiu WU. Hybridoma antibody-based competitive ELISA in Schistosoma japonicum infection. Am J Trop Med Hyg 1983;32(1):114–117.
24. Nascimento E, Tavares CA, Lopes JD. Immunodiagnosis of human cysticercosis (Taenia solium) with antigens purified by monoclonal antibodies. J Clin Microbiol 1987;25(7):1181–1185.
25. Soh CT, et al. Yonsei Rep Trop Med 1985;16:1–10.
26. Tsang VC, Brand JA, Boyer AE. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 1989;159(1):50–59.
27. Tsang VC, Peralta JM, Simons AR. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol 1983;92:377–391.
28. Yong TS, Im K, Chung PR. Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies. Korean J Parasitol 1991;29(3):293–310.
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