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Korean J Parasitol > Volume 31(2):1993 > Article

Original Article
Korean J Parasitol. 1993 Jun;31(2):109-116. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.2.109
Copyright © 1993 by The Korean Society for Parasitology
Worm recovery rate and small intestinal lesions of albino rats coinfected with Fibricola seoulensis and Metagonimus yokogawai
S J Hong,*1H C Woo,1S Y Lee,1J H Ahn,1C K Park,2J Y Chai,3 and S H Lee3
1Department of Parasitology, College of Medicine, Gyeong-Sang National University, Chinju 660-280, Korea.
Received March 29, 1993; Accepted April 29, 1993.


Worm recovery rates and pathologic changes in small intestine of albino rats were observed after concurrent and challenge infections with metacercariae (MC) of Fibricola seoulensis and Metagonimus yokogawai, and compared with those of single infection groups. Albino rats in concurrent infection group were killed 20 days after feeding with 1,000 MC of each fluke. Rats in challenge infection group were fed with 1,000 MC of F. seoulensis and challenged by 1,000 MC of M. yokogawai 10 days after primary infection, then killed 10 days thereafter. In concurrent infection group, mean number of F. seoulensis and M. yokogawai recovered, 250 and 118 respectively, were similar to those of single infection groups. However, more flukes were collected from the duodenum and less flukes were from the ileum than from single infection group. In challenge infection group, the recovery rate of F. seoulensis was similar to that of single infection group and the distribution of the flukes was similar to that of concurrent infection group. Mean number of M. yokogawai, 69, was significantly lower than that of single infection group. Its distribution, however, extended to the duodenum and most of the flukes were recovered from the jejunum. In concurrent infection group, villi of the duodenum were more markedly thickened, fused and shortened than those in F. seoulensis single infection group. The crypt epithelium appeared to be hyperplastic and inflammatory cell infiltration into the villous stroma was mild. Villous atrophy in the jejunum and ileum was milder than in M. yokogawai single infection group. In challenge infection group, the findings were similar to those of concurrent infection group.


Figs. 1-6
Fig. 1-3.F. seoulensis 10-day infection group. Fig. 1. Villi of duodenum were shortened, blunted and edematous. Villus tip beneath the worm (arrow) was eroded and epithelium flattened. (H-E, × 60). Fig. 2. Severe shortening, fusion, blunting and widening of jejunal villi. (H-E, × 100). Fig. 3. Ileum showing moderately thickened, blunted and shortened villi, and moderate crypt hyperplasia. Mild edema and microhemorrhage was recognized in stroma. (H-E, × 40). Fig. 4. In F. seoulensis 20-day infection group, a wide and flat villus of duodenum looked like several villi fusioned into one. (H-E, × 100). Fig. 5. Ileum of M. yokogawai 10-day infection group showing moderate villous atrophy and crypt hyperplasia. (H-E, × 100). Fig. 6. Severely atrophied and edematous villi, and moderate inflammatory cell infiltration in stroma of ileum in M. yokogawai 20-day infection group. (H-E, × 100).

Figs. 7-10
Figs. 7-8. Concurrent infection group of F. seoulensis and M. yokogawai. Fig. 7. A worm is on the flattened villi fused like a syncytium in duodenum. (H-E, × 40). Fig. 8. Moderately thickened and blunt-ended villi of ileum. (H-E, × 100). Figs. 9-10. Challenge infection group of F. seoulensis with M. yokogawai. Fig. 9. Severe inflammatory cell infiltration with eosinophils and neutrophils, was found in severely atrophied duodenal villi. (H-E, × 100). Fig. 10. Villi of ileum showing moderate thickening, fusion, blunting and inflammatory cell infiltration.(H-E, × 40).


Table 1
Worm recovery of Fibricola seoulensis and Metagonimus yokogawai from albino rats

Table 2
Histopathologic changes of rat intestine following infection with F. seoulensis and M. yokogawai

1. Behnke JM, Bland PW, Wakelin D. Effect of the expulsion phase of Trichinella spiralis on Hymenolepis diminuta infection in mice. Parasitology 1977;75(1):79–88.
2. Bruce RG, et al. Parasitology 1977;74:163–173.
3. Chai JY. Seoul J Med 1979;20:104–117.
4. Chai JY, et al. Seoul J Med 1985;26:197–200.
5. Hong SJ, Lee SH, Seo BS, Hong ST, Chai JY. Studies On Intestinal Trematodes In Korea: IX. Recovery Rate And Development Of Fibricola Seoulensis In Experimental Animals. Korean J Parasitol 1983;21(2):224–233.
6. Hong ST. Studies On Intestinal Trematodes In Korea: VII. Growth, Development And Recovery Of Fibricola Seoulensis From Experimentally Infected Rats And Mice. Korean J Parasitol 1982;20(2):112–121.
7. Hong ST, Chai JY, Lee SH. Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus. Korean J Parasitol 1986;24(1):95–97.
8. Hong SJ, Seo BS, Lee SH, Chai JY. A human case of Centrocestus armatus infection in Korea. Korean J Parasitol 1988;26(1):55–60.
9. Howard RJ, Christie PR, Wakelin D, Wilson MM, Behnke JM. The effect of concurrent infection with Trichinella spiralis on Hymenolepis microstoma in mice. Parasitology 1978;77(3):273–279.
10. Huh S, Chai JY, Hong ST, Lee SH. Clinical and histopathological findings in mice heavily infected with Fibricola seoulensis. Korean J Parasitol 1988;26(1):45–53.
11. Kang SY, Cho SY, Chai JY, Lee JB, Jang DH. A Study On Intestinal Lesions Of Experimentally Reinfected Dogs With Metagonimus Yokogawai. Korean J Parasitol 1983;21(1):58–73.
12. Kho WG, et al. Seoul J Med 1990;31:191–199.
13. Lee SH, Yoo BH, Hong ST, Chai JY, Seo BS, Chi JG. A histopathological study on the intestine of mice and rats experimentally infected by Fibricola Seoulensis. Korean J Parasitol 1985;23(1):58–72.
14. Miller HR. The protective mucosal response against gastrointestinal nematodes in ruminants and laboratory animals. Vet Immunol Immunopathol 1984;6(1-2):167–259.
15. Mimori T, Nawa Y, Korenaga M, Tada I. Strongyloides ratti: mast cell and goblet cell responses in the small intestine of infected rats. Exp Parasitol 1982;54(3):366–370.
16. Wakelin D, Lloyd M. Accelerated expulsion of adult Trichinella spiralis in mice given lymphoid cells and serum from infected donors. Parasitology 1976;72(3):307–315.
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