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Korean J Parasitol > Volume 30(2):1992 > Article

Original Article
Korean J Parasitol. 1992 Jun;30(2):151-153. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1992.30.2.151
Copyright © 1992 by The Korean Society for Parasitology
The maintenance of free-living amoebae by cryopreservation
S A Seo,T S Yong and K Im
Department of Parasitology College of Medicine & Institute of Tropical Medicine, Yonsei University, Seoul 120-752, Korea.

We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall 0.7℃/min) than by fast cooling (overall 1.3℃/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophozoites. The survival rate was 2-39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.


Fig. 1
The comparison of cooling rate.

Fig. 2
The recoveries after storage in the liquid nitrogen. The initial suspension of amoevae contained 2×106 trophozoites/ampoule.


Table 1
The comparison of survival rates (%) in the case of 2.5×106 of Naegleria fowleri and Acanthamoeba culbertsoni trophozoites by means of fast cooling and slow cooling

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