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Korean J Parasitol > Volume 30(2):1992 > Article

Original Article
Korean J Parasitol. 1992 Jun;30(2):83-90. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1992.30.2.83
Copyright © 1992 by The Korean Society for Parasitology
Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay
W Y Choi,H W Nam,J H Youn,D J Kim,Y Kong,**S Y Kang,** and S Y Cho**
Department of Parasitology, Catholic University Medical College, Seoul 137-701, Korea.
Abstract

Sensitivity of anti-Toxoplasma antibody (IgG) test by enzyme-linked immunosorbent assay (ELISA) was evaluated in comparison with indirect latex agglutination (ILA) using 2,016 paired human samples of serum and cerebrospinal fluid (CSF). The samples were collected from neurologic patients in Korea with mass lesions in central nervous system (CNS) as revealed by imaging diagnosis (CT/MRI). When the sera were screened for anti-Toxoplasma antibody by ILA, 76 cases(3.8%) were positive (1:32 or higher titers). In the paired samples of CSF, no positive reactions were observed. When ELISA was performed using PBS extract of Percoll purified tachyzoites as antigen, cut-off absorbance was determined as 0.40 for serum and 0.27 for CSF tests. The antibody positive rates by ELISA were 7.0% in serum and 5.6% in CSF. Of them, 40 cases (2.0%) showed positive reactions in both serum and CSF. The antibody positive rates were higher in groups older than 40 years. The rates were higher in male (4.7% by ILA, 8.3% by ELISA) than in female (2.2% by ILA, 5.0% by ELISA). The rates in CSF showed no such sex difference. ELISA showed twice higher positive rates when serum was tested, and was sensitive enough to detect specific antibodies in CSF. Etiologic relations between positive antibody tests and CNS lesions remained unknown.

Figures


Fig. 1
Relations between anti-Toxoplasma antibody (IgG) in sera by ELISA and the ILA titers.


Fig. 2
Relations between anti-Toxoplasma antibody (IgG) in CSF by ELISA and ILA titers.


Fig. 3
Comparison of percent of positive between ILA and ELISA. ▪, serum and □, CSF.


Fig. 4
Correlationship between ELISA values of the sera and CSF among ILA positive cases. ○, 1:32; •, 1:64; □, 1:128; ▪, 1:256; ▵, 1:512 and ▴, 1:1,024 or higher.


Fig. 5
Profile of antibody positive rates by age. ○, sera by ILA; •, sera by ILISA and ▿, CSF by ELISA


Fig. 6
Positive rates of anti-Toxoplasma antibodies by sex. ▪, male and □, female.

Tables


Table 1
Frequencies of serological ILA titers over 1:8


Table 2
ILA titers of CSF of which the serum titers are 1:1,024 or higher


Table 3
Prevalence of ElISA positive cases by ILA titers in 2,016 neurologic patients


Table 4
Screening of sera by ILA for anti-Toxoplasma antibody


Table 5
Screening of sera and CSF for anti-Toxoplasma antibodies ELISA

References
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