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Korean J Parasitol > Volume 30(2):1992 > Article

Original Article
Korean J Parasitol. 1992 Jun;30(2):65-74. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1992.30.2.65
Copyright © 1992 by The Korean Society for Parasitology
Tegumental ultrastructure of juvenile and adult Echinostoma cinetorchis
S H Lee,H S Jun,W M Sohn,* and J Y Chai
Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.

The tegumental ultrastructure of juvenile and adult Echinostoma cinetorchis (Trematoda: Echinostomatidae) was observed by scanning electron microscopy. Three-day (juvenile) and 16-day (adult) worms were harvested from rats (Sprague-Dawley) experimentally fed the metacercariae from the laboratory-infected fresh water snail, Hippeutis cantori. The worms were fixed with 2.5% glutaraldehyde, processed routinely, and observed by an ISI Korea DS-130 scanning electron microscope. The 3-day old juvenile worms were elongated and ventrally curved, with their ventral sucker near the anterior two-fifths of the body. The head crown was bearing 37-38 collar spines arranged in a zigzag pattern. The lips of the oral and ventral suckers had 8 and 5 type II sensory papillae respectively, and between the spines, a few type III papillae were observed. Tongue or spade-shape spines were distributed anteriorly to the ventral sucker, whereas peg-like spines were distributed posteriorly and became sparse toward the posterior body. The spines of the dorsal surface were similar to those of the ventral surface. The 16-day old adults were leaf-like, and their oral and ventral suckers were located very closely. Aspinous head crown, oral and ventral suckers had type II and type III sensory papillae, and numerous type I papillae were distributed on the tegument anterior to the ventral sucker. Scale-like spines, with broad base and round tip, were distributed densely on the tegument anterior to the ventral sucker but they became sparse posteriorly. At the dorsal surface, spines were observed at times only at the anterior body. The results showed that the tegument of E. cinetorchis is similar to that of other echinostomes, but differs in the number and arrangement of collar spines, shape and distribution of tegumenal spines, and type and distribution of sensory papillae.


Figs. 1-5
Fig. 1. Ventral view of a 3-day old juvenile worm of E. cinetorchis (×370).

Fig. 2. Head crown of a 3-day old worm with its oral sucker(CS) and collar spines (arrow heads) (×1,010).

Fig. 3. The ventral sucker of a 3-day old worm showing type II sensory papillae on its lip (×1,020).

Fig. 4. Tegument of ventral surface anterior to the ventral sucker (3-day worm). Tongue of spade-shape apincs and type III sensory papillae (arrow head) are seen (×4,060).

Fig. 5. Tegument of ventral surface posterior to the ventral sucker (30day worm), showing peg-like spines (×4,060).

*Bar unit: µm.

Figs. 6-11
Fig. 6. Velvety tegument of ventro-posterior region of a 3-day worm (×10,200).

Fig. 7. Tegument of dorsal surface anterior to the ventral sucker (3-day worm), showing tongue or spade-shape spines (×4,050).

Fig. 8. Tegument of dorso-median region (3-day worm), showing peg-like spines distributed sparesly (×4,050).

Fig. 9. Tegument of dorsal posterior one-third region (3-day worm), showing nearly no spines (×4,050).

Fig. 10. Tegument of posterior extremity (3-day worm), showing nearly no spines (×10,200).

Fig. 11. Ventral view of a 16-day old adult specimen of E. cinetorchis (×20.4).

*Bar unit: µm.

Figs. 12-17
Fig. 12. Head crown of a 16-day worm, showing collar spines retracted from the tegumental surface (×306).

Fig. 13. Head crown (3-day worm) with protruded collar spines, for comparison with Fig. 12(×830).

Fig. 14. Dorsal view of the head crown (3-day worm), showing partially protruded collar spines (arrow heads) (×2,030).

Fig. 15. Tegument of ventral surface anterior to the ventral sucker (16-day worm), showing scale-like spines and type I sensory papillae (×2,040).

Fig. 16. Another portion, where the spines are retracted from the tegumental surface (×3,060).

Fig. 17. Tegument of ventro-lateral region between the head crown and ventral sucker (16-day worm), where 1~4 groups of type I papillae are scatteredly seen (×1,020).

*Bar unit: µm.

Figs. 18-22
Fig. 18. Ventral sucker, cirrus (C) and genital opening (GO), a 16-day worm (×407).

Fig. 19. Lip of the ventral sucker (16-day worm), showing the wrinkled tegument and many type III sensory papillae(×1,530).

Fig. 20. Tegument of ventro-posterior region of a 16-day worm. The tegument became smooth and the spines are distributed very sparsely (×800).

Fig. 21. Tegument of corso-anterior region (16-day worm), showing velvety cytoplasmic processes with few spines (×3,060).

Fig. 22. Tegument of dorso-posterior region (16-day worm), showing porous and cobblestone-like tegumental surface (×6,020).

*Bar unit: µm.

1. Ahn YK, Ryang YS, Chai JY, Sohn WM. [Cercarial shedding of Echinostoma cinetorchis and experimental infection of the cercariae to several kinds of snails]. Korean J Parasitol 1989;27(3):203–211.
2. Ando R, et al. Dubutsugaku Zasshi 1923;35:108–119.
3. Bennett CE. Scanning electron microscopy of Fasciola hepatica L. during growth and maturation in the mouse. J Parasitol 1975;61(5):892–898.
4. Chai JY, Lee SH. Intestinal trematodes of humans in Korea: Metagonimus, heterophyids and echinostomes. Korean J Parasitol 1990;28 Suppl:103–122.
5. Cho SY, Kang SY, Ryang YS. [Helminthes Infections In The Small Intestine Of Stray Dogs In Ejungbu City, Kyunggi Do, Korea]. Korean J Parasitol 1981;19(1):55–59.
6. Font WF, Wittrock DD. Scanning electron microscopy of Leucochloridiomorpha constantiae during development from metacercaria to adult. J Parasitol 1980;66(6):955–964.
7. Fried B, Fujino T. Scanning electron microscopy of Echinostoma revolutum (Trematoda) during development in the chick embryo and the domestic chick. Int J Parasitol 1984;14(1):75–81.
8. Fujino T, Ishii Y, Cho DW. Surface ultrastructure of the tegument of Clonorchis sinensis newly excysted juveniles and adult worms. J Parasitol 1979;65(4):579–590.
9. Hyodo, et al. Jpn J parasitol 1958;25(4):262–273.
10. Kawahara S, et al. Tokyo Iji Shinshi 1933;2840:1794–1796.
11. Koie M. Stereoscan studies of cercariae, metacercariae, and adults of Cryptocotyle lingua (Creplin 1825) Fischoeder 1903 (Trematoda: Heterophyidae). J Parasitol 1977;63(5):835–839.
12. Lee SH, Chai JY, Hong ST, Sohn WM. Experimental life history of Echinostoma cinetorchis. Korean J Parasitol 1990;28(1):39–44.
13. Lee SH, Hong SJ, Chai JY, Hong ST, Seo BS. [Tegumental ultrastructures of Echinostoma hortense observed by scanning electron microscopy]. Korean J Parasitol 1986;24(1):63–70.
14. Lee SH, et al. Seoul J Med 1985;26(1):52–63.
15. Lee SH, Hong ST, Seo BS. [A Study On The Fine Tegumental Structures Of The Metacercaria And Juvenile Stages Of Clonorchis Sinensis]. Korean J Parasitol 1982;20(2):123–132.
16. Lee SH, Kim SJ, Chai JY, Sohn WM. [Tegumental ultrastructures of Paragonimus iloktsuenensis according to the developmental stages]. Korean J Parasitol 1989;27(1):57–66.
17. Lee SH, Lee JK, Sohn WM, Hong ST, Hong SJ, Chai JY. Metacercariae of Echinostoma cinetorchis encysted in the fresh water snail, Hippeutis(Helicorbis) cantori, and their development in rats and mice. Korean J Parasitol 1988;26(3):189–197.
18. Lee SH, Seo BS, Chai JY, Hong SJ. [Study on Metagonimus yokogawai(Katsurada, 1912) in Korea VII. Electron microscopic observation on the tegumental structure]. Korean J Parasitol 1984;22(1):1–10.
19. Lee SH, Sohn WM, Hong ST. Scanning electron microscopical findings of Echinochasmus japonicus tegument. Korean J Parasitol 1987;25(1):51–58.
20. Lee SK, Chung NS, Ko IH, Ko HI, Sohn WM. [A case of natural human infection by Echinostoma cinetorchis]. Korean J Parasitol 1988;26(1):61–64.
21. Lumsden RD. Surface ultrastructure and cytochemistry of parasitic helminths. Exp Parasitol 1975;37(2):267–339.
22. Moriyana S. Nippon Kiseichu Gakkai Kiji 1952;21:132.
23. Ryang YS, Ahn KY, Kim WT, Shin KC, Lee KW, Kim TS. [Two cases of human infection by Echinostoma cinetorchis]. Korean J Parasitol 1986;24(1):71–76.
24. Seo BS, et al. Seoul J Med 1980;21(1):21–29.
25. Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies On Parasitic Helminths Of Korea 5.Survey On Intestinal Trematodes Of House Rats. Korean J Parasitol 1981;19(2):131–136.
26. Seo BS, Lee SH, Chai JY, Hong ST, Hong SJ. [Studies on intestinal trematodes in Korea X. Scanning electron microscopic observation on the tegument of Fibricola seoulensis]. Korean J Parasitol 1984;22(1):21–29.
27. Seo BS, Park YH, Chai JY, Hong SJ, Lee SH. [Studies On Intestinal Trematodes In Korea Xiv. Infection Status Of Loaches With Metacercariae Of Echinostoma Cinetorchis And Their Development In Albino Rats]. Korean J Parasitol 1984;22(2):181–189.
28. Seo BS, Rim HJ, Lee CW. Studies on the parasitic helmiths of Korea: I. Trematodes of rodents. Korean J Parasitol 1964;2(1):20–26.
29. Smales LR, et al. J Helminthol 1984;58:187–195.
30. Takahashi S, et al. Tokyo Iji Shinshi 1930;2657:141–144.
31. Takahashi S, et al. Tokyo Iji Shinshi 1930;2678:1326–1327.
32. Threadgold LT. The Ultrastructure of the "cuticle" of Fasciola hepatica. Exp Cell Res 1963;30:238–242.
33. Threadgold LT. Quarterly J Microscop Sci 1963;104:505–512.
34. Threadgold LT. Electron-microscope studies of Fasciola heaptica. 3. Further observations on the tegument and associated structures. Parasitology 1967;57(4):633–637.
35. Yamashita J. Progress of Med Parasit 1964;1:287–313.
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