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Korean J Parasitol > Volume 30(1):1992 > Article

Original Article
Korean J Parasitol. 1992 Mar;30(1):25-31. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1992.30.1.25
Copyright © 1992 by The Korean Society for Parasitology
Immunohistochemical localization of 36 and 29 kDa proteins in sparganum
L S Kim,Y Kong,S Y Kang and S Y Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.

Antigenic proteins of 36 and 29 kDa were localized in Spirometra mansoni plerocercoid (sparganum) immunohistochemically by avidin biotin complex (ABC) staining. When polyclonal antibodies such as BALB/c mouse serum immunized with crude saline extract of sparganum or confirmed sparganosis sera were reacted as primary antibodies, the positive chromogen (3-amino, 9-ethylcarbazole) reactions were recognized at syncytial tegument, tegumental cells, muscle and parenchymal cells and lining cells of excretory canals. A monoclonal antibody (MAb) which was reacting to 36 and 29 kDa proteins in the extract of the worm was localized at the syncytial tegument and tegumental cells. The present results suggested that the potent antigenic proteins of 36 and 29 kDa in sparganum were produced at the tegumental cells and transported to the syncytial tegument.


Fig. 1
SDS-PAGE/immunoblot finding of SP-53 monoclonal antibody. In SDS-PAGE, a linear gradient gel of 10~15% was used for protein separation in reducing condition.

Figs. 2-9
Fig. 2. Nagative control in ABC staining (×100). A 1:1000 diluted healthy human serum was used as a primary antibody. Positive reactions were not observed in any tissue.

Fig. 3. Another negative control (×100). Uninfected mouse serum was reacted at dilution of 1:100 as a primary antibody instead of human serum. No structures were stained with AEC chromogen.

Fig. 4. Positive control (×100). A hyperimmune BALB/c mouse serum was reacted as a primary antibody. Syncytial tegument, tegumental cells, parenchymal cells, excretory canals and muscle cells were stained positively.

Fig. 5. Another positive control of immunized BALB/c mouse serum (×200). Tegument, parenchymal cells and excretory canals were stained with AEC chromogen.

Fig. 6. When a 1:200 diluted patient serum was reacted, strong positive reactions were observed at surface of syncytial tegument, tegumental cells, a part of muscle cells, parenchymal cells and excretory canals (×100).

Fig. 7. Staining with another patient serum (×200). Stronger reactions were shown at surface of syncytial tegument and tegumental cells.

Fig. 8. MAb reacting to 36 and 29 kDa was used as a primary antibody (×100). Surface of syncytial tegument and tegumental cells showed strong positive reactions.

Fig. 9. Another example of ABC staining treated with 36 and 29 kDa MAb (×400). Strong positive reactions at syncytial tegument and tegumental cells were observed.

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