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Korean J Parasitol > Volume 29(4):1991 > Article

Original Article
Korean J Parasitol. 1991 Dec;29(4):389-396. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.4.389
Copyright © 1991 by The Korean Society for Parasitology
Changes of IgM and IgG antibody levels in experimental rabbit anisakiasis as observed by ELISA and SDS-PAGE/immunoblot
H J Yang,Y J Cho and Y H Paik
Department of Parasitology, College of Medicine, Kyung-Hee University, Seoul 130-701, Korea.

Antibody changes in experimental anisakiasis were observed in 10 rabbits which were infected each with 10 Anisakis simplex larvae. The sera were collected before and on the 6th to the 95th day after the infection. Using crude saline extract of Anisakis larvae as antigen, specific IgM and IgG antibody levels were observed by ELISA and SDS-polyacrylamide gel electrophoresis/immunoblot. Levels of specific-IgM antibody were elevated from the 6th day, reached their peaks on the 11th day after the infection, and dropped thereafter. Serum levels of IgG antibody increased from the 6th day and reached their peak on the 26th day after the infection, and decreased gradually thereafter. When SDS-PAGE of the crude extract was done, at least forty-one SDS-polypeptide bands were recognized. Of them, IgM antibody reacted mainly to the bands of 168, 95, 74, 64, 51, 47 and 34 kDa while IgG antibody reacted strongly to 168, 92, 85, 64, 58, 52, 42 and 40 kDa bands. The crude extract showed negligible cross reactions with sera of other parasitic diseases and normal control.


Fig. 1
Changes of the individual absorbance of Anisakis-specific IgM antibody in experimental rabbit anisakiasis.

Fig. 2
Anisakis-specific IgG antibody levels in rabbit sera of experimental anisakiasis.

Fig. 3
Changes of mean absorbance of Anisakis specific-IgM(+) and -IgG(▪) antibody levels (mean±S.D.) in infected rabbit sera.

Fig. 4
SDS-PAGE/immunoblot finding of IgM reacting bands. In SDS-PAGE, 7.5~15% gradient separating gels were used. Numericals at top of the figure indicated the postinfection days. Mr: Molecular mass in kDa, C: Amidoblack B stained NC slip of the crude extract, P: Preinfection.

Fig. 5
Finding of IgG reacting bands in SDS-PAGE/immunoblot. In SDS-PAGE, 7.5~15% gradient separating gels were used. Numericals at top of the figure indicated the postinfection days. Mr: Molecular mass in kDa, C: Amidoblack B stained NC slip of the crude extract, P: Preinfection.


Table 1
Serum levels of Anisakis-specific IgG antibody in other parasitic infections and control

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