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Korean J Parasitol > Volume 29(3):1991 > Article

Original Article
Korean J Parasitol. 1991 Sep;29(3):267-277. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.3.267
Copyright © 1991 by The Korean Society for Parasitology
Natural killer cell activity in Naegleria fowleri infected mice
K R Lee,C O Shin and K Im
Department of Parasitology, College of Medicine and Institute of Tropical Medicine, Yonsei University, Seoul 120-752, Korea.
Abstract

The natural killer (NK) cell activity of splenocytes and recycling capacity of NK cells were observed by combining the 51Cr-release cytotoxicity assay and single cell cytotoxicity assay against YAC-1.

The ICR mice were infected intranasally with Naegleria fowleri, that is a pathogenic free-living amoeba. The mice infected with 1 × 105 trophozoites showed mortality rate of 76.7% and mean survival time of 12.9 days.

The cytotoxic activity of NK cells in infected mice was significantly higher than that of non-infected mice during the period between 12 hours and day 3 after infection, and highest on day 1. The target-binding capacity of NK cells in infected mice was not different from that of non-infected ones. Maximal killing potential and maximal recycling capacity were remarkably increased in infected mice as compared with the control.

The results obtained in this observation indicated that elevated NK cell activity in mice infected with N. fowleri was not due to target-binding capacity of NK cells but due to the increased activity of NK cells and increased recycling capacity of individual NK cells.

Figures


Fig. 1
Data from Table 1 are used to exemplify the calculation of Vmax. Initial number of target cells(T) is poltted against number of dead target cells(V) at the end of the test. The points fit well to the theoretical Michaelis-Menten saturation curve. A Line-weaver-Burk polt is used to calculate Vmax and Km. 1/T=X plotted against 1/V=Y. Data are taken from Table 1 and Fig. 1. Regression analysis is used to obtain a straight line (r=0.990). Vmax equals the reciprocal of the Y intercept and Km equals the negative reciprocal of the X intercept.


Fig. 2
Natural killer cell cytotoxic activity against YAC-1 target cells in mice infected with Naegleria fowleri.


Fig. 3
Single cell cytotoxic activity against YAC-1 target cells in mice infected with Naegleria fowleri.


Fig. 4
Vmax against YAC-1 target cell in mice infected with Naegleria fowleri.


Fig. 5
Maximal recycling capacity against YAC-1 target cells in mice infected with Naegleria fowleri.

Tables


Table 1
A representative experiment showing data used from calculations of Vmax and Km


Table 2
Cumulative number of death in mice inoculated intranasally with Naegleria fowleri


Table 3
NK activity, Vmax, TBC, fraction of dead conjugates, and estimation of active NK & their maximal recycling capacity*


Table 4
Correlation matrix between Vmax, TBC, active NK, MRC and NK activity

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