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Korean J Parasitol > Volume 29(2):1991 > Article

Original Article
Korean J Parasitol. 1991 Jun;29(2):149-159. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.2.149
Copyright © 1991 by The Korean Society for Parasitology
Isolation and identification of Cryptosporidium from various animals in Korea. II. Identification of Cryptosporidium muris from mice
J K Rhee,Y S Seu and B K Park
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju 560-756, Korea.

Each of SPF mice(Scl: ICR strain, 3-week-old males) was inoculated with 5 × 104 oocysts of Cryptosporidium by stomach tube. The oocysts were large type one which was previously isolated from Korean mice, and passaged in 3-week-old SPF mice. The patterns of oocyst discharge were monitored daily, and in order to observe the ultrastructure of developmental stages the stomach of the mice was examined by transmission electron microscopy (TEM) at 4 weeks post-inoculation. The prepatent period for 6 mice was 5.6 days post-inoculation on the average, and the patent period was 63.2 days. The number of oocysts discharged per day from the mice reached peak on day 36.6 post-inoculation on the average. A large number of oocysts were found in fecal samples obtained from inoculated mice on days 30-50 post-inoculation. C. muris was larger than C. parvum at almost every developmental stages, the size difference being 1.4 times in oocysts, 2.4 times in sporozoites, 1.6 times in merozoites, and 1.5 times in microgametes. The ultrastructural features of the attachment site of C. muris to the mucus cells were remarkably different from those of C. parvum and its closely related species. The anterior projection of the protozoa (C. muris), the outer aspect of which was surrounded by a thick filamentous process of the host cell, has not been reported at any developmental stages of C. parvum or its closely related species. The size of the oocysts of strain RN 66 was larger than that of Korean mice origin. The above results reveal that the large type Cryptosporidium of Korean mice origin is identified as Cryptosporidium muris and this type was named as C. muris (strain MCR).


Fig. 1
Pattern of oocyst discharge in mice inoculate with 2.5×104 large type of Cryptosporidium oocysts (Korean mice origin).

Figs. 2-3
Fig. 2. A younger trophozoite growing in the microvillus of the surface mucus cell(MC) of the stomach, ×23,000.

Fig. 3. A trophozoite showing the feeder organelle(FO) and annular ring(A). Filamentous process(F) of the host cell surrounds the feeder organelle of the parasite. Dense band(D) separates the parasite area from the main cytoplasm of the host cell. The Golgi complex(GO) appeared to be composed fo smooth endoplasmic reticulum, ×16,000.

Figs. 4-5
Fig. 4. A trophozoite showing mitochondria (MI), Golgi Complex(GO) and rough and smooth endo-plasmic reticulum, ×28,000.

Fig. 5. A meront showing merozoites(ME) and residual body(REB), ×22,000.

Figs. 6-7
Fig. 6. Longitudinal section of merozoites(ME) showing nucleus, micronemes(MN) and longitudinal ridges(arrows) in the pellicle, ×17,000.

Fig. 7. Transverse section of merozoites showing micronemes(MN), subpellicular microtubules(arrows) and longitudinal ridges(triangles) in th pellicle, ×37,000.

Figs. 8-9
Fig. 8. A microgamont comprising microgametes. Longitudinal section showing microtubules(arrows) and expanded anterior part(triangle), ×20,000.

Fig. 9. A younger macroganete showing numerous amylopectin bodies(AP) and refractile body(RB), ×12,000.

Figs. 10-11
Fig. 10. A macrogamete showing numerous amylopectin bodies (AP) and refractile body(RB), x16,000.

Fig. 11. An oocyst at the late stage of sporulation showing 4 sporozoites(SP), oocyst wall(W), residual body(REB) and refractile body(RB), ×20,000.

Figs. 12-13
Nomarski interference-contrast photomicrographs of developmental stage of Cryptosporidium muris in mucosal scrapings of the stomach of experimentally infected adult mice. Mature oocysts(MO) showing residual bodies, amylopectin-like granules and lipid-like granules. R, red blood cell; W, oocyst wall. Both figures ×1,500.


Table 1
Patent period and peak time of oocyst discharge in mice inoculated with large type Cryptosporidium oocysts from Korean mice origin

Table 2
Dimensions of large type Cryptosporidium oocysts between Korean mice origin and strain RN 66 (Osmic acid pre-fixed Giemsa staining)

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