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Korean J Parasitol > Volume 28(3):1990 > Article

Original Article
Korean J Parasitol. 1990 Sep;28(3):155-160. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.3.155
Copyright © 1990 by The Korean Society for Parasitology
Histochemical findings of the tribocytic organ and tegument of Fibricola seoulensis
S Huh,S H Lee,* and B S Seo*
Department of Parasitology, College of Medicine, Hallym University, Chunchon 200-702, Korea.
*Department of Parasitology, Seoul National University College of Medicine, Seoul 110-460, Korea.

The tribocytic organ and tegument of Fibricola seoulensis were examined histochemically for the detection of carbohydrates, mucosubstances, amyloid, collagen and alkaline phosphatase. The surface, secretes, gland cells of the tribocytic organ, and the tegument of the worms were positive to periodic acid Schiff (PAS) and PAS with diastase stain but negative to other stains. It was inferred that the tribocytic organ and tegument of F. seoulensis comprise neutral mucopolysaccharides, which may take a protective role against host enzymes. The surface and secretes of the tribocytic organ, and the tegument of the worms were also positive to double bridge PAP for alkaline phosphatase. This fact suggests that they may play a role as both self protective and host tissue lytic functions.


Figs. 1-9
Fig. 1. PAS reaction. Positive-red, G: gland cells of the tribocytic organ, S: secretes of the gland, TE: tegument of the worm, ×140.

Fig. 2. PAS reaction with diastase control. Positive-red, G:gland cells, S: secretes of the gland, ×140.

Fig. 3. Alcian blue (pH2.5) stain. Positive-blue, G: gland cells, ×100.

Fig. 4. Toluidine blue stain. Metachromatic substance-red, T: tribocytic organ, ×140.

Fig. 5. Aldehyde fuchsin stain. Sulfated MPS-deep purple violet, T: tribocytic organ, ×140.

Fig. 6. Sudan black B stain. Glycolipid-bluish black, T: tribocytic organ, ×140.

Fig. 7. Congo red stain. Amyloid-red, T: tribocytic organ, ×140.

Fig. 8. Gomori's rapid one step trichrome stain. Collagen-greenish blue, G: gland cells, ×140.

Fig. 9. Double bridge PAP for alkaline phosphatase. Positive-golden yellow, G: gland cells, TE: tegument, ×40.


Table 1
Results of stains

Table 2
Staining reactions of carbohydrates and mucosubstances

Table 3
Staining reactions of amyloid and collagen

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