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Korean J Parasitol > Volume 28(3):1990 > Article

Original Article
Korean J Parasitol. 1990 Sep;28(3):143-154. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.3.143
Copyright © 1990 by The Korean Society for Parasitology
Cell-mediated immunity in mice infected with Acanthamoeba culbertsoni
M J Kim,C O Shin and K I Im
Department of Parasitology, College of Medicine & Institute of Tropical Medicine, Yonsei University, Seoul 120-752, Korea.

Observations were made on the differences of cell-mediated responses in mice of three infection groups differently scheduled in their severity with pathogenic Acanthamoeba culbertsoni. Infections were done by dropping 5 µl saline suspension containing 3 × 103, 1 × 104, or 1 × 105 trophozoites, respectively. Amoebae were cultured axenically in CGV medium and inoculated into the right nasal cavity of C3H/HeJ mice aging around 6-8 weeks, under the anesthesia by intraperitoneal injection of secobarbital.

Delayed type hypersensitivity (DTH) responses in footpad and blastogenic responses of mouse spleen cells using (3H)-thymidine and the serum antibody titer were measured up to day 14 after infection, and natural killer cell activities were measured up to day 5 after infection. The results obtained in this study were as follows:

1. The mice infected with 3 × 103 trophozoites showed mortality rate of 17%, and 34% in the mice infected with 1 × 104 trophozoites and 65% with 1 × 105 trophozoites.

2. In regard to DTH responses in all experimental groups, the level increased on day 7 and declined on day 14 after infection, but their differences could not be noted between infected and control groups.

3. The blastogenic responses of splenocytes treated with amoeba lysates and lipopolysaccharides (LPS) showed no difference from the control group. The blastogenic responses of splenocytes treated with concanavalin A were declined significantly in the experimental group as compared with the control group, but the blastogenic responses of splenocytes treated with polyinosinic acid were not different from the control group. There was also no difference among three infected groups.

4. The cytotoxic activity of the natural killer cells was activated on day 1 after infection and declined to the level of control group on day 2 in all experimental groups. On day 5 after infection, the natural killer cell cytotoxicity was significantly suppressed as compared with the control groups.

5. The serum antibody titers of the infected mice increased after day 7, but there was no statistical difference between the three infected groups.

In summary of the results, there was no difference in cell-mediated immune responses of three experimental groups scheduled with different infection intensities. But there was a significant difference in cell-mediated immune responses between infected and control mice. It is considered that cell-mediated immune responses should be involved in murine model infected with A. culbertsoni.


Fig. 1
Survival rates of mice inoculated intranasally with A. culbertsoni.

Fig. 2
Pattern of footpad swelling with A. culbertsoni lysates in mice infected with the amoeba.

Fig. 3
Blastogenic response (stimulation index) of con. A or poly I treated splenocytes in A. culbertsoni infected mice and non-infected (control) mice.

Fig. 4
Natural killer cell cytotoxicity against YAC-1 target cells in mice infected with A. culbertsoni.

Fig. 5
Circulating antibody titers in mice infected with A. culbertsoni.


Table 1
Cumulative number of death in mice inoculated untranasally with A. culbertsoni

Table 2
Elicitation of footpad swelling with A. culbertsoni lysates in mice infected with the amoeba.

Table 3
Blastogenic response (stimulation index) of the splenocytes treated with amoeba lysates in mice infected with A. culbertsoni and non-infected (control) mice

Table 4
Blastogenic response (stimulation index) of the splenocytes treated with LPS in mice infected with A. culbertsoni and non-infected (control) mice

Table 5
Blastogenic response (stimulation index) of the splenocytes treated with con. A or poly I in mice infected with 3×103 trophozoites of A. culbertsoni and non-infected (control) mice

Table 6
Blastogenic response (stimulation index) of the splenocytes treated with con. A or poly I in mice infected with 1×104 trophozoites of A. culbertsoni and non-infected (control) mice

Table 7
Blastogenic response (stimulation index) of the splenocytes treated with con. A or poly I in mice infected with 1×105 trophozoites of A. culbertsoni and non-infected (control) mice

Table 8
Natural killer cell cytotoxicity against YAC-1 target cells in mice infected with Acanthamoeba culbertsoni

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