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Korean J Parasitol > Volume 28(2):1990 > Article

Original Article
Korean J Parasitol. 1990 Jun;28(2):101-108. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.2.101
Copyright © 1990 by The Korean Society for Parasitology
Component proteins in cystic fluid of Taenia solium metacestodes collected surgically from neurocysticercosis patients
Y Kong,S Y Kang and S Y Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Abstract

Surgically collected cystic fluid of Taenia solium metacestodes from patients of intracranial cystic lesion were compared in their protein composition with those from naturally infected pigs in Cheju Do, Korea and Ecuador. In non-denaturing discontinuous-polyacrylamide gel electrophoresis (disc-PAGE), no discernible differences were recognized in banding patterns between the cystic fluids from Cheju Do and Ecuador, and between the cystic fluids from pigs and human lesions except wider bands that corresponded to human albumin and γ-globulin (in 4 of 9 patients). In reducing SDS-PAGE, bands in the cystic fluid from Ecuador showed the same banding pattern with that from Cheju Do but two bands of 21 and 17 kDa were stained darker. Cystic fluids from patients revealed the same protein compositions of the major protein bands of 94, 64, 15, 10 and 7 kDa as in the cystic fluid of pig origin, but human albumin (66 kDa), heavy and light chains of gamma globulin (55 and 22.5 kDa) were contaminated in 4 of 9 cystic fluids. Human CSF proteins seem to have been contaminated during cystic fluid collection. In any cystic fluid from patients, the major protein component was 150 kDa which was subdivided into 15, 10 and 7 kDa in reducing SDS-PAGE.

Figures


Fig. 1
Disc-PAGE findings of cystic fluid(CF) from pigs and human, and normal DSF at 7.5% gel concentration. 1: CF from Ecuador, 2: CF from Cheju Do, 3: HSC*-895, 4: HSC-4953, 5: HSC-802, 6: HSC-1692, 7: HSC-1702, 8: HSC-2976, 9: HSC-5718, 10: HSC-2922, 11: HSC-7310, 12: Normal CFS

*Our reference file of Human Serum Collection



Fig. 2
SDS-PAGE findings of CF from pigs and human and normal human CSF. Linear gradient gel of 10-15% was used for protein separation. Mr: Molecular weight in kDa. Lane 1-12: Same as described in Fig. 1. ⇦: Human albumin ←: Heavy chain of γ-globulin ⇐:Light chain of γ-globulin ◁: Unidentified band

Tables


Table 1
Protein content and result of antigenicity of cystic fluid which were collected surgically from human brain lesions

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