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Korean J Parasitol > Volume 27(3):1989 > Article

Original Article
Korean J Parasitol. 1989 Sep;27(3):217-223. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1989.27.3.217
Copyright © 1989 by The Korean Society for Parasitology
Scanning electron microscopic observations of Thelazia callipaeda from human
W Y Choi,J H Youn,H W Nam,W S Kim,W K Kim,S Y Park,* and Y W Oh**
Catholic Institute of Parasitic Diseases, Catholic University Medical College, Seoul 137-701, Korea.
*Park Sang Yun Eye Clinic, Seoul 150-010, Korea.
**Oh Young Whan Eye Clinic, Seoul 135-040, Korea.

Four females and a male nematode isolated from 2 patients who visited eye clinics in Seoul were identified as Thelazia callipaeda and their ultrastructures were observed by scanning electron microscopy (SEM). General features of the worms were slender and attenuated at both ends. Vaginal opening was located at 0.27 mm from the anterior end, and in front of the esophago-intestinal junction. In the body cuticle transverse striations varied characteristically through the body. The number of cuticular transverse striations was 400-650/mm at head portion, 250/mm at middle portion and 300-350/mm at tail portion. The SEM observation of the mouth part of the females showed 6 cord-like cuticular thickenings in hexagonal arrangement and an amphid was observed. A lateral line, a vaginal opening, a pair of phasmids, and an anus were identified in the body portion. A pair of papillae and 6 cord-like cuticular thickenings were on the mouth part of the male. It was difficult to observe structures at the tail of the male except wrinkle-like structures. Most of the larvae isolated from the uterus of a female worm were sheathed and thus cuticular striations were not seen. Others were un-sheathed and revealed cuticular striations. The oval membrane which encysted sheathed larvae was also observed. These are the 18th and 19th record of human thelaziasis in Korea as the literature are concerned.


Figs. 1-11
Scanning electron micrographs of female T. callipaeda. (bar ; µm)

Fig. 1. Head portion of adult female. (×430)

Fig. 2. Cord-like cuticular thickenings (CD) at the margin of mouth. (×1500)

Fig. 3. Amphid (AM) on the outside of mouth. (×3000)

Fig. 4. Vaginal opening. (×4400)

Fig. 5. Lateral line in middle part. (×2000)

Fig. 6. Tail en face view. PH; phasmid. (×1000)

Fig. 7. Phasmid opening. (×2000)

Fig. 8. anus. (×1500)

Figs. 9-11. Variation of cuticular transverse striations.

Fig. 9. Striations at anterior part. (×1800)

Fig. 10. Striations at miccle part. (×1800)

Fig. 11. Striations at posterior part. (×2000)

Figs. 12-20
Scanning electron micrographs of male T. callipaeda. (bar; µm)

Fig. 12. Mouth part showing a pair of head papilla(P). (×1000)

Fig. 13. Cord-like cuticular thickenings at the margin of mouth opening. (×3000)

Fig. 14. Wrinkle-like structures (W) at the tail. (×1000)

Fig. 15. Cuticular striations at middle part. (×1000)

Figs. 16-20. Scanning electron micrographs of larvae. (bar; µm)

Fig. 16. Sheathed larva. (×1200)

Fig. 17. Higher magnification of Fig. 16. (×4000)

Figs. 18-19. Unsheathed larva showing cuticular striations. SH; sheath. (Fig. 18:×7200; Fig. 19:×4000)

Fig. 20. Oval membrane(OV). (×220)


Table 1
Measurement data of the worms (dimension: mm)

1. Arizono N, et al. Jpn J Parasitol 1976;25(5):402–408.
2. Cha IS, et al. J Korean Ophthalmol Soc 1983;24(3):651–654.
4. Choi DK, et al. . J Korean Ophthalmol Soc 1978;19(1):125–129.
5. Choi WY, Lee WK, Lee OR, Shin HH, Shin NY. A Case Of Human Thelaziasis In Seoul. Korean J Parasitol 1977;15(2):127–132.
6. Hong ST, Lee SH, Han H. A Human case of thelaziasis in Korea. Korean J Parasitol 1985;23(2):324–326.
7. Hong ST, Lee SH, Kim SI. A human case of Thelazia callipaeda infection with reference to its internal structures. Korean J Parasitol 1988;26(2):137–140.
8. Hong ST, Lee SH, Shim YB, Choi JS, Choe JK. [A Human Case Of Thelaziasis In Korea]. Korean J Parasitol 1981;19(1):76–80.
9. Im KI, Ahn MH, Kim JJ, Kim HB. [A Case Of Human Thelaziasis In Korea]. Korean J Parasitol 1982;20(1):60–63.
10. Kagei N, et al. . Jpn J Parasitol 1983;32(5):481–484.
11. Lee BS, et al. . J Korean Ophthalmol Soc 1986;27(1):85–89.
12. Min HK, Chun KS. [A case of human thelaziasis occurred in both eyes]. Korean J Parasitol 1988;26(2):133–135.
13. Nagada K. Jpn J Parasitol 1964;13(7):600–602.
14. Oh CK, et al. J Korean Ophthalmol Soc 1975;16(4):431–434.
15. Ryu JS, Im KI, Byun YJ, Kim SC. A case of human thelaziasis in Korea. Korean J Parasitol 1987;25(1):83–84.
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