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Korean J Parasitol > Volume 27(2):1989 > Article

Original Article
Korean J Parasitol. 1989 Jun;27(2):73-77. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1989.27.2.73
Copyright © 1989 by The Korean Society for Parasitology
Cell-mediated immunity in experimental amoebic meningoencephalitis
K I Im,P R Chung and T U Kim
Department of Parasitology, College of Medicine, and Institute of Tropical Medicine, Yonsei University, Seoul 120-753, Korea.

Cell-mediated and humoral immune reactions in mice infected with pathogenic Acanthamoeba culbertsoni were observed according to the period of time after amoebic infection by intranasal inoculation. The degrees of blastogenesis of spleen cells induced by mitogens, which were measured using radioactive [3H]-thymidine, were compared between infected and non-infected control groups. The mitogens used in this blastogenesis experiment were concanavalin A (Con A) and lipopolysaccharide(LPS). On the other hand, enzyme-linked immunosorbent assay(ELISA) was employed for the detection of humoral antibodies against A. culbertsoni. The levels of blastogenesis of splenocytes and serum titres in the experimental group showed increasing tendency a week after inoculation of A. culbertsoni, although there was no difference between the experimental and control groups in other periods of the experimental time.


Fig. 1
Survival rate of mice infected intranasally with A. culbertsoni.

Fig. 2
Stimulation index(blastogenesis) of mitogen-treated splenocytes from non-infected(•…•) and A. culbertsoni infected(∘…∘) mice.

Fig. 3
Changes of circulating antibody titers as measured by ELISA in A. culbertsoni-infected(∘…∘) and control(•…•) mice.


Table 1
Stimulation index* (blastogenic response) of mitogen-treated splenocytes from non-infected and A. culbertsoni-infected mice

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