| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasitol > Volume 26(4):1988 > Article

Original Article
Korean J Parasitol. 1988 Dec;26(4):245-254. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1988.26.4.245
Copyright © 1988 by The Korean Society for Parasitology
Imunological characterization of antigens from cysticercus and sparganum and their application to immunodiagnosis 1. Immunological characteristics of crude antigenic components from Cysticercus cellulosae
Chang Hwan Kim and James Yang*
College of Natural Science, Gyeongsang National University, Chinju 660-701, Korea.
*Tropical Diseases Unit, Toronto General Hospital, Toronto, Canada.
Abstract

We studied the serological reaction between various antigenic components from Cysticercus cellulosae and IgG antibodies in sera of cysticercosis, sparganosis, hydatidosis patients and normal humans by ELISA and EITB. In serological tests by ELISA, we recognized cross reaction of Cysticercus antigenic components with IgG antibodies in heterologous sera such as sparganosis and hydatidosis patients or normal humans. The crude antigenic components of Cysticercus showed lower ELISA sensitivity in homologous sera from cysticercosis patients than heterologous sera from hydatidosis patients. A total of 31 polypeptide bands with 260 KDa-22 KDa molecular weights were detected by SDS-PAGE, and 11 of them showed strong intensity. Total 22 components of them were recognized by IgG antibodies in cysticercosis patients sera. However, 12 of them were recognized also by normal human sera, 11 were by sparganosis sera, and 21 were by hydatidosis patients sera. The crude antigenic components of 104 KDa, 82 KDa, 72 KDa, 59 KDa and 34 KDa molecular weights were nonspecific ones, which cross-reacted with sera of either cysticercosis, sparganosis, hydatidosis patients or normal humans.

Figures


Fig. 1
Extraction procedure of crude antigenic protein from Cysticercus.


Fig. 2
Procedure of ELISA.


Fig. 3
Resolved bands of crude antigenic protein extracted from C. cellulosae by 12% SDS-PAGE.

(A: Protein was visualized by staining with Coomassie blue R-250, B: Protein was visualized by staining with silver)

[HM: High molecular weight marker protein, LM: Low molecular weight marker protein, BPB: Bromophenol blue, Cy: Cysticercus crude antigenic protein]



Fig. 4
Antigenic components from Cysticercus reacting with sera of 7 cysticercosis patients.

(M: Marker protein, Cn: Serial No. of cysticercosis sera)



Fig. 5
Antigenic components reacting with sera of 7 normal humans (control group).

(M: Marker protein, Nn: Serial No. of normal sera)



Fig. 6
Antigenic components reacting with sera of 4 sparganosis patients.

(M: Marker protein, Sn: Serial No. of sparganosis sera)



Fig. 7
Antigenic components reacting with sera of 9 hydatidosis patients.

(M: Marker protein, Hn: Serial No. of hydatidosis sera)


Tables


Table 1
The ELISA absorbance values obtained in patients sera using Cysticercus crude extract as antigen (data of 7, 5, 9 sera of each disease and 7 controls)


Table 2
Crude antigenic proteins from Cysticercus reacting with cysticercosis, sparganosis, hydatidosis and normal sera by EITB

References
1. Arambulo PV 3rd, Walls KW, Bullock S, Kagan IG. Serodiagnosis of human cysticercosis by microplate enzyme-linked immunospecific assay (ELISA). Acta Trop 1978;35(1):63–67.
 
2. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–254.
3. Cho SY, Kim SI, Kang SY, Choi DY, Suk JS, Choi KS, Ha YS, Chung CS, Myung HJ. Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean J Parasitol 1986;24(1):25–41.
 
4. Choi BK, et al. Chung-Ang J Med 1986;11(2):135–146.
5. Coker-Vann M, Brown P, Gajdusek DC. Serodiagnosis of human cysticercosis using a chromatofocused antigenic preparation of Taenia solium cysticerci in an enzyme-linked immunosorbent assay (ELISA). Trans R Soc Trop Med Hyg 1984;78(4):492–496.
  
6. Costa JM, Ferreira AW, Makino MM, Camargo ME. Spinal fluid immunoenzymatic assay (ELISA) for neurocysticercosis. Rev Inst Med Trop Sao Paulo 1982;24(6):337–341.
 
7. Diwan AR, Coker-Vann M, Brown P, Subianto DB, Yolken R, Desowitz R, Escobar A, Gibbs CJ Jr, Gajdusek DC. Enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to cysticerci of Taenia solium. Am J Trop Med Hyg 1982;31(2):364–369.
 
9. Estrada JJ, Kuhn RE. Immunochemical detection of antigens of larval Taenia solium and anti-larval antibodies in the cerebrospinal fluid of patients with neurocysticercosis. J Neurol Sci 1985;71(1):39–48.
  
10. Flisser A, Porez-Montfort R, Larralde C. The immunology of human and animal cysticercosis: a review. Bull World Health Organ 1979;57(5):839–856.
 
11. Gottstein B, Tsang VC, Schantz PM. Demonstration of species-specific and cross-reactive components of Taenia solium metacestode antigens. Am J Trop Med Hyg 1986;35(2):308–313.
 
12. Grogl M, Estrada JJ, MacDonald G, Kuhn RE. Antigen-antibody analyses in neurocysticercosis. J Parasitol 1985;71(4):433–442.
  
17. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680–685.
  
18. Larralde C, Laclette JP, Owen CS, Madrazo I, Sandoval M, Bojalil R, Sciutto E, Contreras L, Arzate J, Diaz ML, et al. Reliable serology of Taenia solium cysticercosis with antigens from cyst vesicular fluid: ELISA and hemagglutination tests. Am J Trop Med Hyg 1986;35(5):965–973.
 
19. Miller B, Goldberg MA, Heiner D, Myers A, Goldberg A. A new immunologic test for CNS cysticercosis. Neurology 1984;34(5):695–697.
  
20. Mohammad IN, Heiner DC, Miller BL, Goldberg MA, Kagan IG. Enzyme-linked immunosorbent assay for the diagnosis of cerebral cysticercosis. J Clin Microbiol 1984;20(4):775–779.
 
21. Morrissey JH. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 1981;117(2):307–310.
  
22. Oakley BR, Kirsch DR, Morris NR. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 1980;105(2):361–363.
  
23. Pammenter MD, Rossouw EJ. The value of an antigenic fraction of Cysticercus cellulosae in the serodiagnosis of cysticercosis. Ann Trop Med Parasitol 1987;81(2):117–123.
 
24. Park CG. J Army Med Officers Corp 1958;6:69–71.
25. Plancarte A, et al. . Child's Nerv Syst 1987;3:203–205.
26. Proctor EM, Powell SJ, Elsdon-Dew R. The serological diagnosis of cysticercosis. Ann Trop Med Parasitol 1966;60(2):146–151.
 
27. Rim HJ, Song KW, Joo KH, Lee JS, Kim JJ. [An Epidemiological Note On The Taeniasis In Korea]. Korean J Parasitol 1980;18(2):235–240.
 
28. Carbajal JR, Palacios E, Azar-Kia B, Churchill R. Radiology of cysticercosis of the central nervous system including computed tomography. Radiology 1977;125(1):127–131.
 
29. Rodriguez-Carbajal J, Salgado P, Gutierrez-Alvarado R, Escobar-Izquierdo A, Aruffo C, Palacios E. The acute encephalitic phase of neurocysticercosis: computed tomographic manifestations. AJNR Am J Neuroradiol 1983;4(1):51–55.
 
30. Schantz PM, Shanks D, Wilson M. Serologic cross-reactions with sera from patients with echinococcosis and cysticercosis. Am J Trop Med Hyg 1980;29(4):609–612.
 
31. Schleicher M, et al. Anal Biochem 1983;31:312–317.
 
32. Téllez-Girón E, Ramos MC, Dufour L, Alvarez P, Montante M. Detection of Cysticercus cellulosae antigens in cerebrospinal fluid by dot enzyme-linked immunosorbent assay (Dot-ELISA) and standard ELISA. Am J Trop Med Hyg 1987;37(1):169–173.
33. Towbin H, Gordon J. Immunoblotting and dot immunobinding--current status and outlook. J Immunol Methods 1984;72(2):313–340.
  
34. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 1979;76(9):4350–4354.
  
35. Tsang VC, Hancock K, Maddison SE, Beatty AL, Moss DM. Demonstration of species-specific and cross-reactive components of the adult microsomal antigens from Schistosoma mansoni and S. japonicum (MAMA and JAMA). J Immunol 1984;132(5):2607–2613.
 
36. Tsang VC, Peralta JM, Simons AR. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol 1983;92:377–391.
  
37. Voller A, Bidwell DE, Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ 1976;53(1):55–65.
 
38. WHO. Research needs in taeniasis-cysticercosis. Bull World Health Organ 1976;53(1):67–73.
39. Yakoleff-Greenhouse V, Flisser A, Sierra A, Larralde C. Analysis of antigenic variation in cysticerci of Taenia solium. J Parasitol 1982;68(1):39–47.
  
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
Share:      
METRICS
6
Crossref
1
Scopus
3,725
View
38
Download
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2022 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI