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Korean J Parasitol > Volume 26(1):1988 > Article

Original Article
Korean J Parasitol. 1988 Mar;26(1):39-44. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1988.26.1.39
Copyright © 1988 by The Korean Society for Parasitology
Immunization effect on Naegleria fowleri infection in splenectomized mice
Kwang Hyub Han,Myoung Hee Ahn,* and Duk Young Min*
Department of Internal Medicine, Yonsei University College of Medicine, Seoul 120-749, Korea.
*Department of Parasitology, Hanyang University College of Medicine, Seoul 133-791, Korea.

A pathogenic free-living amoeba, Naegleria fowleri, is a causative protozoan parasite of primary amebic meningoencephalitis in human and experimental animals. It is known that humoral and cellular immunity contribute as the defence mechanism of host against this organism. Recently splenectomy has been argued on its effect on host defence mechanisms. The present study was aimed to observe the effect of immunization in splenectomized mice. For immunization, 5-10 × 10(5) trophozoites of Naegleria fowleri o 359 were intraperitoneally inoculated once a week for two weeks to BALB/c mice, and 5-10 × 10(4) of ameba trophozoites were intranasally inoculated for infection after splenectomy and/or immunization. ELISA technique was applied for the detection of serum IgG antibody levels. Experimental animals were divided into 4 groups; I. splenectomized and immunized; II. splenectomized only; III. immunized only; IV. not splenectomized nor immunized. The results obtained were as follows: Mortality rates of splenectomized and immunized mice in group I (38.1 percent) and immurized only in group III (25.0 percent) were lower than those of not immunized mice in group II(50 percent) and control group, IV (46.4 percent). Survival times of mice in group I, II, III and IV were 20.1±3.6, l7.3±4.5, 20.4±7.0 and 19. 6±7.6 days respectively, and there were no significant differences between them. ELISA values (absorbance at 492 nm) of group I (1.10±0.29) and group III (1.31±0.28) were signficantly higher than that of group IV(0.24±0.37) at day 31 of infection (p<0.05). Conclusively, it is presumed that humoral immunity against N. flowleri may operate as ever, after immunization, even though the mouse was splenectomized.


Fig. 1
Death rates of mice infected with N. fowleri after splenectomy and/or immunization. (Group I: splenectomized and immunized, Group II: splenectomized, Group III: immunized, Group IV: not splenectomized nor immunized)


Table 1
Mortality and mean survival time of N. fowleri infected mice after splenectomy and/or immunization

Table 2
Serum IgG antibody levels measured by ELISA technique after immunization and/or infection of N. fowleri in mice*

1. Aaberge IS, Heier HE, Hem E, Giercksky KE, Groeng EC. IgM and IgG response to pneumococcal polysaccharide vaccine in normal individuals and individuals splenectomized due to trauma. Acta Pathol Microbiol Immunol Scand [C] 1984;92(1):11–16.
2. Adams AC, John DT, Bradley SG. Modification of resistance of mice to Naegleria fowleri infections. Infect Immun 1976;13(5):1387–1391.
4. Carter RF. Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis, and of the experimental pathological changes induced by it. J Pathol 1970;100(4):217–244.
5. Chaimoff C, Douer D, Pick IA, Pinkhas J. Serum immunoglobulin changes after accidental splenectomy in adults. Am J Surg 1978;136(3):332–333.
6. Derrick EH. Trans Roy Soc Trop Med Hyg 1948;42:191–198.
7. Ferrante A, et al. Immunology 1980;2:37–41.
8. Francke EL, Neu HC. Postsplenectomy infection. Surg Clin North Am 1981;61(1):135–155.
9. Ghadirian E, Meerovitch E. Effect of splenectomy on the size of amoebic liver abscesses and metastatic foci in hamsters. Infect Immun 1981;31(2):571–573.
10. Haggerty RM, John DT. Serum agglutination and immunoglobulin levels of mice infected with Naegleria fowleri. J Protozool 1982;29(1):117–122.
11. Hosea SW, Burch CG, Brown EJ, Berg RA, Frank MM. Impaired immune response of splenectomised patients to polyvalent pneumococcal vaccine. Lancet 1981;1(8224):804–807.
12. Im KI, Oh HS. [Immunological Tests By Anti-Free-Living Amoebas Serum Produced In Experimental Animals: I. Immobilization Of Free-Living Amoebas In Vitro By Rabbit Antiserum]. Korean J Parasitol 1978;16(1):41–46.
14. Koren A, Haasz R, Tiatler A, Katzuni E. Serum immunoglobulin levels in children after splenectomy. A prospective study. Am J Dis Child 1984;138(1):53–55.
15. Lockwood CM. Immunological functions of the spleen. Clin Haematol 1983;12(2):449–465.
16. Martinez J, Duma RJ, Nelson EC, Moretta FL. Experimental naegleria meningoencephalitis in mice. Penetration of the olfactory mucosal epithelium by Naegleria and pathologic changes produced: a light and electron microscope study. Lab Invest 1973;29(2):121–133.
17. Oster CN, Koontz LC, Wyler DJ. Malaria in asplenic mice: effects of splenectomy, congenital asplenia, and splenic reconstitution on the course of infection. Am J Trop Med Hyg 1980;29(6):1138–1142.
18. Park KM, Ryu JS, Im KI. [Blastogenic responses of splenic lymphocytes to Naegleria fowleri lysates and T-cell mitogen in mice with primary amoebic meningoencephalitis]. Korean J Parasitol 1987;25(1):1–6.
19. Reilly MF, White KL Jr, Bradley SG. Host resistance of mice to Naegleria fowleri infections. Infect Immun 1983;42(2):645–652.
20. Rosner F, Zarrabi MH, Benach JL, Habicht GS. Babesiosis in splenectomized adults. Review of 22 reported cases. Am J Med 1984;76(4):696–701.
21. Ruben FL, Hankins WA, Zeigler Z, Norden CW, Harrison A, Winkelstein A, Herrmann DJ. Antibody responses to meningococcal polysaccharide vaccine in adults without a spleen. Am J Med 1984;76(1):115–121.
22. Shin HJ, Im KI, Choe RS. [Effect of splenectomy on development of primary amoebic meningoencephalitis]. Korean J Parasitol 1985;23(1):156–164.
23. Sullivan JL, Ochs HD, Schiffman G, Hammerschlag MR, Miser J, Vichinsky E, Wedgwood RJ. Immune response after splenectomy. Lancet 1978;1(8057):178–181.
24. Thong YH, Shepherd C, Ferrante A, Rowan-Kelly B. Protective immunity to Naegleria fowleri in experimental amebic meningoencephalitis. Am J Trop Med Hyg 1978;27(2 Pt 1):238–240.
25. Thong YH, Ferrante A, Shepherd C, Rowan-Kelly B. Resistance of mice to Naegleria meningoencephalitis transferred by immune serum. Trans R Soc Trop Med Hyg 1978;72(6):650–652.
26. Voller A, Bidwell DE, Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ 1976;53(1):55–65.
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