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Korean J Parasitol > Volume 25(2):1987 > Article

Original Article
Korean J Parasitol. 1987 Dec;25(2):154-158. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1987.25.2.154
Copyright © 1987 by The Korean Society for Parasitology
The karyotype of Paramphistomum cervi (Zeder, 1790) from Korean cattle
Jae Ku Rhee,Yong Hwan Kim and Bae Keun Park
Department of Veterinary Parasitology, Jeonbug National University, Jeonbug 520, Korea.

As a series of systematic classification of paramphistomes, the worms in the rumen and reticulum were collected on 214 Korean cattle slaughtered at Jeonju abattoir from January 1986 to April 1987 and were classified by means of morphology. Afterwards, the karyotype of Paramphistomum cervi (Zeder, 1790) was detected by means of modified air-drying method from germ cells of the worms. The results were summarized as follows: In the chromosome number of 254 P. cervi, the haploid cell was n=9 and the diploid 2n=18. The meiotic divisions were observed frequently; 1,924 haploid and 32 diploid cells were reliable. Nine pairs of mitotic chromosomes were homologous in the metaphase stage, and the chromosomes were composed of five medium-sized metacentrics (m), subtelocentrics (st) or submetacentrics (sm) and four small-sized subtelocentrics(st) or submetacentrics(sm). Meiotic metaphase was composed of five medium and four small chromosomes in size. As a series of C-banding method, C-band was showed in centromeric region from all of the haploid germ cells. Whereas chromosome No. 3 and 5 included heterochromatin on the tip region, chromosome No. 4 on the distal region and No. 6 proximal region. And chromosomes No. 2 and 8 showed a remarkable C-band distinguished from other chromosomes.


Fig. 1
The mitotic chromosomes(18) in cultured germ cells of paramphistomum cervi prepared by modified air dry method.

Fig. 2
The meiotic chromosomes(9) in cultured germ cells of paramphistomum cervi prepared by modified air dry method.

Fig. 3
The karyotype plate made from the mitotic metaphase of paramphistomum cervi.

A: Medium size B: Small size

Fig. 4
The meiotic chromosomes(haploid) of paramphistomum cervi.

A: Conventional karyotype of germ cell B: C-banded karyotype of germ cell


Table 1
Chromosome measurements and their classification in Paramphistomum cervi

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