| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasitol > Volume 25(2):1987 > Article

Original Article
Korean J Parasitol. 1987 Dec;25(2):110-122. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1987.25.2.110
Copyright © 1987 by The Korean Society for Parasitology
Histopathological changes of the liver after praziquantel treatment in Clonorchis sinensis infected rabbits
Soon Hyung Lee,Sung Tae Hong,Chong Soon Kim,Woon Mok Sohn,Jong Yil Chai and Yoon Seong Lee*
Department of Parasitology, College of Medicine, Seoul National University, Seoul 110, Korea.
*Department of Forensic Medicine and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110, Korea.
Abstract

The rabbits were infected with Clnorchis sinensis and were treated with praziquantel at the dose of 50 mg/kg × 2 × 2 days afer 1, 2, 4, 8 weeks and 7 months from the infection. Their livers were observed histopathologically 1, 4 and 12 weeks after treatment. The findings are summarized as below: The changes of the liver in control rabbits were relatively mild until 2 weeks after infection. However, widening and thickening of bile ducts, proliferation of biliary epithelium and periductal fibrosis were moderate after 4 weeks from infection and those changes were severe after 8 weeks and 7months. Goblet cell metaplasia was found after 8 weeks from infection. The mild changes of 2-week infection group were completely recovered by 4 weeks after the treatment. In the groups of 4 or more weeks after infection, the changes of bile ducts became milder in the degree after the treatment, but were still found 12 weeks after the treatment. As the infection duration was passed, more severe changes were observed after the treatment. In this context, it is concluded that the liver changes of acute clonorchiasis in the early two weeks are reversible by treatment while chronic biliary epithelial changes are irreversible. Therefore, early treatment should be recommended as possible to minimize the remaining histopathological changes of liver in clonorchiasis.

Figures


Figs. 1-4
Fig. 1. Infected control of 1 week infection showing a juvenile C. sinensis in a small bile duct, and periductal edema, hematoxylin-eosin (HE) stained, ×140.

Fig. 2. Two large ducts after 4 weeks from treatment of 1 week infection with slight periductal inflammation, HE stained, ×100.

Fig. 3. Infected control of 2 week infection showing a worm in a dilated bile duct. Papillary proliferation of the duct epithelia and periductal edema and inflammation were observed, HE stained, ×40.

Fig. 4. High power view of a bile duct 12 weeks after treatment of 2 week infection with slight periductal inflammation, HE stained, ×200.



Figs. 5-8
Fig. 5. Infected control of 4 week infection, 2 sections of mature worms were found in a dilated bile duct. Biliary epithelial layer was compressed, flattened or desquamated near the worms, HE stained, ×48.

Fig. 6. Small bile ducts 1 week after treatment of 4 week infection with slight epithelial proliferation and periductal fibrosis, HE stained, ×72.

Fig. 7. A large bile duct 12 weeks after treatment of 4 week infection showing slight epithelial proliferation and periductal inflammation, HE stained, ×48.

Fig. 8. Three sections of C. sinensis in a severely dilated bile duct after 8 week from infection. Epithelial proliferation and compression, desquamation and periductal inflammation were observed, HE stained, ×40.



Figs.9-12
Fig. 9. A large bile duct a without worm of a 8 week infected control rabbit, with papillary or glandular proliferation of epithelium, HE stained, ×40.

Fig. 10. A large duct one week after treatment of 8 week infection with irregular epithelial proliferation and periductal fibrosis, HE stained, ×40.

Fig. 11. A large duct 4 weeks after treatment of 8 week infection with irregular epithelial proliferation and periductal fibrosis, HE stained, ×56.

Fig. 12. A large duct 12 weeks after treatment of 8 week infection with slight epithelial proliferation and periductal fibrosis, HE stained, ×56.



Figs. 13-16
Fig. 13. A sectioned worm of mature C. sinensis in a dilated bile duct was found 7 months after infection. Epithelial layer of the duct was proliferated markedly and desquamated partly, HE stained, ×40.

Fig. 14. A large duct with papillary and adenomatous hyperplasia of epithelium and periductal fibrosis and inflammation, of 7 month infection, HE stained, ×40.

Fig. 15. High power of a bile duct 7 months infected control showing goblet cells in biliary epithelium, HE stained, ×200.

Fig. 16. A large bile duct 4 weeks after treatment of 7 month infection showing papillary and adenomatous hyperplasia and periductal fibrosis, HE stained, ×56.


Tables


Table 1
Number of rabbits infected by C. sinensis* and treated with praziquantel**


Table 2
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 1 week duration of infection


Table 3
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 2 week duration of infection


Table 4
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 4 week duration of infection


Table 5
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 8 week duration of infection


Table 6
Histopathological scoring* of liver in rabbit clonorchiasis after treatment in 7 month duration of infection

References
1. el-Hawey AM, Hassan I, el-Ibiary S, Massoud AM. Histopathological changes in experimental schistosomiasis mansoni before and after praziquantel therapy. J Egypt Soc Parasitol 1986;16(1):117–126.
 
2. Chang HP. Pathological Changes In The Intrahepatic Bile Ducts Of Cats (Felis Catus) Infested With Clonorchis Sinensis. J Pathol Bacteriol 1965;89:357–364.
  
3. Hutradilok N, Ruenwongsa P, Thamavit W, Upatham ES. Liver collagen in Opisthorchis viverrini infected hamsters following praziquantel treatment. Southeast Asian J Trop Med Public Health 1983;14(3):290–293.
 
4. Kang TW. Seoul Univ J 1963;14:49–79.
5. Komiya Y. Clonorchis and clonorchiasis. Adv Parasitol 1966;4:53–106.
  
6. Lee SH. Large scale treatment of Clonorchis sinensis infections with praziquantel under field conditions. Arzneimittelforschung 1984;34(9B):1227–1230.
 
7. Lee SH, Shim TS, Lee SM, Chi JG. [Studies On Pathological Changes Of The Liver In Abino Rats Infected With Clonorchis Sinensis]. Korean J Parasitol 1978;16(2):148–155.
 
8. Lee SY, Lee SH, Chi JG. [Ultrastructural Changes Of The Hepatocytes And Biliary Epithelia Due To Clonorchis Sinensis In Guinea Pigs]. Korean J Parasitol 1978;16(2):88–102.
 
9. Mehlhorn H, Frenkel JK, Andrews P, Thomas H. Light and electron microscopic studies on Schistosoma mansoni Granulomas of mouse livers following treatment with praziquantel. Tropenmed Parasitol 1982;33(4):229–239.
 
10. Min HK, et al. Ewha Med 1985;8(2):111–115.
11. Rim HJ, Lyu KS, Lee JS, Joo KH. Clinical evaluation of the therapeutic efficacy of praziquantel (Embay 8440) against Clonorchis sinensis infection in man. Ann Trop Med Parasitol 1981;75(1):27–33.
 
12. Seo BS, Lee SH, Chai JY, Hong ST. Praziquantel(Distocide(R)) In Treatment Of Clonorchis Sinensis Infection. Korean J Parasitol 1983;21(2):241–245.
 
13. Seo BS, Lee SH, Cho SY, Chai JY, Hong ST, Han IS, Sohn JS, Cho BH, Ahn SR, Lee SK, Chung SC, Kang KS, Shim HS, Hwang IS. An Epidemiologic Study On Clonorchiasis And Metagonimiasis In Riverside Areas In Korea. Korean J Parasitol 1981;19(2):137–150.
 
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2022 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI