| Home | E-Submission | Sitemap | Contact us |  
Korean J Parasitol > Volume 35(1):1997 > Article

Original Article
Korean J Parasitol. 1997 Mar;35(1):47-54. English.
Published online Mar 20, 1997.  http://dx.doi.org/10.3347/kjp.1997.35.1.47
Copyright © 1997 by The Korean Society for Parasitology
Effects of anti-IgE mAb on serum IgE, FcεRII/CD23 expression on splenic B cells and worm burden in mice infected with Paragonimus westermani
M H Shin,* and H K Min
Department of Parasitology, College of Medicine, Ewha Womans University, Seoul 158-056, Korea.

It is generally accepted that parasite-specific IgE plays a crucial role in host defense against helminthic parasites. However, the role of high levels of nonspecific IgE in helminthic infections is still controversial. To investigate the role of nonspecific IgE in primary infections with P. westermani, the effect of anti-IgE mAb treatment on serum IgE, FcεRII/CD23 expression and worm burden in Paragonimus-infected mice were examined. In mice treated with anti-IgE antibody, the total IgE levels were not detectable (1 µg/ml) throughout the experiment compared with untreated infected mice. The mean percentages of FcεRII/CD23 positive splenic B cells in anti-IgE treated mice (range: 20.3 - 30.5) were also decreased throughout the experiment compared with untreated infected mice (range: 35.7 - 44.4). Reduction of the total IgE and expression of FcεRII/CD23 on splenic B cells resulted in decreased worm burden six weeks post infection. These results suggest that high levels of nonspecific IgE in mice with primary infections of P. westermani play a harmful, rather than beneficial, role for the host, perhaps by interfering with CD23-dependent cellular pathways.


Fig. 1
Effect of anti-IgE mAb on total serum IgE levels in BALB/c mice infected with P. westermani. Total serum IgE levels were determined by ELISA using monoclonal rat anti-mouse IgE.

Fig. 2
Effect of anti-IgE mAb in parasite-specific IgE levels in BALB/c mice infected with P. westermani. Absorbance (O.D) was measured at 450 mn. Asterisk (*) denotes significant difference (P<0.05) between infected mice treated with PBS and infected mice treated with anti-IgE mAb.

Fig. 3
Suppressive effect of anti-IgE on the expression of FcεRII/CD23 on splenic B cell in mice infected with P. westermani. FL1-H and FL2-H indicate green fluorescence (CD23) and red fluorescence (CD45R/B220). respectively. Results ard shown as two color contour plots (A, autofluorescence; B, non-infected mice; C, infected mice treated with PFB; D, infected mice treated with anti-IgE mAb).


Table 1
Effect of anti-IgE in the precent (%) of FcεRII/CD23 positive splenic B cells in mice infected with Paragonimus westermani

Table 2
Effect of anti-IgE on the number of worms recovered in mice infected with Paragonimus westermani

1. Amiri P, Haak-Frendscho M, Robbins K, McKerrow JH, Stewart T, Jardieu P. Anti-immunoglobulin E treatment decreases worm burden and egg production in Schistosoma mansoni-infected normal and interferon gamma knockout mice. J Exp Med 1994;180(1):43–51.
2. Auriault C, Damonneville M, Verwaerde C, Pierce R, Joseph M, Capron M, Capron A. Rat IgE directed against schistosomula-released products is cytotoxic for Schistosoma mansoni schistosomula in vitro. Eur J Immunol 1984;14(2):132–138.
3. Capron M, Spiegelberg HL, Prin L, Bennich H, Butterworth AE, Pierce RJ, Ouaissi MA, Capron A. Role of IgE receptors in effector function of human eosinophils. J Immunol 1984;132(1):462–468.
4. Capron M, Jouault T, Prin L, Joseph M, Ameisen JC, Butterworth AE, Papin JP, Kusnierz JP, Capron A. Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages. J Exp Med 1986;164(1):72–89.
5. Delespesse G, et al. Immunol Rev 1992;125:77–79.
6. Flores-Romo L, Shields J, Humbert Y, Graber P, Aubry JP, Gauchat JF, Ayala G, Allet B, Chavez M, Bazin H, et al. Inhibition of an in vivo antigen-specific IgE response by antibodies to CD23. Science 1993;261(5124):1038–1041.
7. Fujiwara H, Kikutani H, Suematsu S, Naka T, Yoshida K, Yoshida K, Tanaka T, Suemura M, Matsumoto N, Kojima S, et al. The absence of IgE antibody-mediated augmentation of immune responses in CD23-deficient mice. Proc Natl Acad Sci U S A 1994;91(15):6835–6839.
8. Haak-Frendscho M, Robbins K, Lyon R, Shields R, Hooley J, Schoenhoff M, Jardieu P. Administration of an anti-IgE antibody inhibits CD23 expression and IgE production in vivo. Immunology 1994;82(2):306–313.
9. Hagan P, Blumenthal UJ, Dunn D, Simpson AJ, Wilkins HA. Human IgE, IgG4 and resistance to reinfection with Schistosoma haematobium. Nature 1991;349(6306):243–245.
10. Ikeda T, Fujita K. IGE in Paragonimus ohirai-infected rats: relationship between titer, migration route, and parasite age. J Parasitol 1980;66(2):197–204.
11. Jones RE, Finkelman FD, Hester RB, Kayes SG. Toxocara canis: failure to find IgE receptors (Fc epsilon R) on eosinophils from infected mice suggests that murine eosinophils do not kill helminth larvae by an IgE-dependent mechanism. Exp Parasitol 1994;78(1):64–75.
12. Kawabe T, Takami M, Hosoda M, Maeda Y, Sato S, Mayumi M, Mikawa H, Arai K, Yodoi J. Regulation of Fc epsilon R2/CD23 gene expression by cytokines and specific ligands (IgE and anti-Fc epsilon R2 monoclonal antibody). Variable regulation depending on the cell types. J Immunol 988;141(4):1376–1382.
13. Bettler B, Hofstetter H, Rao M, Yokoyama WM, Kilchherr F, Conrad DH. Molecular structure and expression of the murine lymphocyte low-affinity receptor for IgE (Fc epsilon RII). Proc Natl Acad Sci U S A 1989;86(19):7566–7570.
14. Kigoni EP, Elsas PP, Lenzi HL, Dessein AJ. IgE antibody and resistance to infection II Effect of IgE suppression on the early and late skin reaction and resistance of rats to Schistosoma mansoni infection. Eur J Immunol 1986;16(6):589–595.
15. Korenaga M, Watanabe N, Tada I. Effects of anti-IgE monoclonal antibody on a primary infection of Strongyloides ratti in mice. Parasitol Res 1991;77(4):362–363.
16. Luo HY, Hofstetter H, Banchereau J, Delespesse G. Cross-linking of CD23 antigen by its natural ligand (IgE) or by anti-CD23 antibody prevents B lymphocyte proliferation and differentiation. J Immunol 1991;146(7):2122–2129.
17. Min DY, Ahn MH, Kim KM, Leem MH, Park SY. [The effects of antibodies and complement in macrophage-mediated cytotoxicity on metacercariae of the lung fluke, Paragonimus westermani]. Korean J Parasitol 1990;28(2):91–100.
18. Min DY, Ryu JS, Shin MH. Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with Paragonimus westermani. Korean J Parasitol 1993;31(3):231–238.
19. Pestel J, Joseph M, Dessaint JP, Capron A. Variation in the expression of macrophage Fc epsilon receptors in relation to experimental rat schistosome infection. Int Arch Allergy Appl Immunol 1988;85(1):55–62.
20. Pirron U, Schlunck T, Prinz JC, Rieber EP. IgE-dependent antigen focusing by human B lymphocytes is mediated by the low-affinity receptor for IgE. Eur J Immunol 1990;20(7):1547–1551.
21. Pritchard DI. Immunity to helminths: is too much IgE parasite--rather than host-protective. Parasite Immunol 1993;15(1):5–9.
22. Radermerker M, Bekhti A, et al. Int Arch Allergy Immnuol 1974;47:285–295.
23. Rousseaux-Prevost R, Capron M, Bazin H, Capron A. IgE in experimental schistosomiasis. II. Quantitative determination of specific IgE antibodies against S mansoni: a follow-up study of two strains of infected rats Correlation with protective immunity. Immunology 1978;35(1):33–39.
24. Shin MH, Min DY. Production of interferon-gamma and interleukin-4 by splenocytes in mice infected with Paragonimus westermani. Korean J Parasitol 1996;34(3):185–189.
25. Shin MH, Ryu JS, Min DY. [Serum IgE levels in rats infected with Paragonimus westermani]. Korean J Parasitol 1991;29(4):397–401.
26. Vouldoukis I, Riveros-Moreno V, Dugas B, Ouaaz F, Becherel P, Debre P, Moncada S, Mossalayi MD. The killing of Leishmania major by human macrophages is mediated by nitric oxide induced after ligation of the Fc epsilon RII/CD23 surface antigen. Proc Natl Acad Sci U S A 1995;92(17):7804–7808.
27. Watanabe N, Katakura K, Kobayashi A, Okumura K, Ovary Z. Protective immunity and eosinophilia in IgE-deficient SJA/9 mice infected with Nippostrongylus brasiliensis and Trichinella spiralis. Proc Natl Acad Sci U S A 1988;85(12):4460–4462.
Editorial Office
c/o Department of Medical Environmental Biology
Chung-AngUniversity College of Medicine, Dongjak-gu, Seoul 06974, Korea
Tel: +82-2-820-5683   Fax: +82-2-826-1123   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2019 by The Korean Society for Parasitology and Tropical Medicine. All rights reserved.     powerd by m2community