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Korean J Parasitol > Volume 24(1):1986 > Article

Original Article
Korean J Parasitol. 1986 Jun;24(1):63-70. Korean.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1986.24.1.63
Copyright © 1986 by The Korean Society for Parasitology
Tegumental ultrastructures of Echinostoma hortense observed by scanning electron microscopy
Soon Hyung Lee,Sung Jong Hong,Jong Yil Chai,Sung Tae Hong and Byong Seol Seo
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110, Korea.
Abstract

The tegumental ultrastructures of Echinostoma hortense adults were observed by scanning electron microscopy. The worms of 4 weeks of age were harvested from albino rats experimentally infected with the metacercariae obtained from the loach. The results were as follows: The worms were leaf-like and their anterior end portion, including oral sucker and head crown, ventrally curved to face posteriorly. The tegument of whole body was wrinkled transversely and covered with cobblestone-like cytoplasmic processes. The oral sucker had roundly swollen (type II) sensory papillae on the ventral half of its lip and uni-ciliated knob-like (type I) sensory papillae, arranged in 2-3 rows, on the dorsal outer surface. Aspinous ventral sucker had many of type I papillae arranged in a circular band on its outer surface. The tegument around the genital opening was of similar feature to the ventral sucker, but sensory papillae were hardly found around the former. Scale-like spines with broad base and round tip were distributed densely on the tegument anterior to the ventral sucker but they became sparse in posterior half of the ventral surface, finally to disappear at posterior extremity. A few number of type I papillae were observed on the ventral surface. The results suggest that the tegument of E. hortense is similar to that of other echinostomes especially E. revolutum. But the number and arrangement of collar spines, and/or the type and distribution of sensory papillae seem characteristic features of E. hortense differed from other echinostomes.

Figures


Figs. 1-6
Fig. 1. Ventral view of 4-week old E. hortense. Bar=300µm.

Fig. 2. Its head part showing oral sucker, head crown, and 27 collar spines including end group ones. Note the arrangement of sensory papillae on the lip of oral sucker. Bar=60µm.

Fig. 3. Ciliated knob-like papillae (type I) aligned in 2~3 rows in the manner of "zigzag" on the outer surface of oral sucker. Bar=3µm.

Fig. 4. Dorso-median part of head crown showing several collar spines covered with square-shaped or rectangular cytoplasmic processes. Bar=7µm.

Fig. 5. Ciliated knob-like (type I) papillae (arrow heads) grouped on the tegument of the root part of end group spines. Bar=10µm.

Fig. 6. Spines on the tegument behind ventral sucker, which are arranged transversely and in "X" manner. Bar=40µm.



Figs. 7-12
Fig. 7. Tegumental surface around genital opening and ventral sucker where is free of trgumental spine. Bar=15µm.

Fig. 8. An erected cirrus protruding out from genital opening. Bar=20µm.

Fig. 9. Spines on the tegument between oral and ventral suckers which are arranged transversely and "X"-like pattern. Non-ciliated round swellings (type II papillae: arrow heads) on the lip of oral sucker are observed. Bar=50µm.

Fig. 10. Scale-like spines with round tip and broad base on the dorso-anterior tegument behind head crown. Bar=10µm.

Fig. 11. More sparsely distributed spines on the postero-ventral surface than in Fig. 10. Bar=10µm.

Fig. 12. Magnification of ciliated knob-like (type I) papillae on the tegument behind ventral sucker. Bar=2µm. Insert: A lateral view enlarged 2 times.


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