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Korean J Parasitol > Volume 25(2):1987 > Article

Original Article
Korean J Parasitol. 1987 Dec;25(2):159-167. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1987.25.2.159
Copyright © 1987 by The Korean Society for Parasitology
Analysis of antigen specificity using monoclonal and polyclonal antibodies to Cysticercus cellulosae by enzyme-linked immunoelectrotransfer blot technique
Seung-Yull Cho,Shin Yong Kang and Suk Il Kim
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 151, Korea.
Abstract

To analyse the antigen specificity of patients sera from 24 confirmed neurocysticercosis and a monoclonal antibody, SDS-PAGE using 10-15% linear gradient gel and EITB were done. Cystic fluid, saline extracts of scolex and of whole worm of C. cellulosae, saline extracts of sparganum, hydatid cyst fluid, saline extracts of Fasciola, Clonorchis and Paragonimus were used as antigen. Of protein bands in cystic fluid of C. cellulosae, patient sera reacted frequently to bands of 152, 94, 64, 48, 24, 15, 10 and 7 kDa proteins. To saline extracts of scolex and whole worm of C. cellulosae, patients sera reacted frequently to 94, 64, 52, 39, 34, 15 and 10 kDa bands. Two bands in sparganum extract (130 and 64 kDa) and two bands in hydatid cyst fluid (52 and 27 kDa) were cross-reacting bands with sera from cysticercosis patient. Saline extracts of Fasciola, Clonorchis and Paragonimus did not exhibit cross-reacting bands. Monoclonal antibody to cystic fluid of C. cellulosae was found to react with low molecular weight proteins of 15, 10 and 7 kDa.

Figures


Fig. 1
SDS-PAGE of 8 antigens in 10~15% linear gradient gel, stained with 0.125% Coomassie brilliant blue R-250. Mr: Standard protein markers, 1: saline extract of sparganum, 2: hydatid cyst fluid, 3: cystic fluic of C. cellulosae, 4: saline extract of Fasciola, 5: saline extract of Clonorchis, 6: saline extract of Paragonimus, 7: saline extract of scolex of C. cellulosae and 8: saline extract of whole worm of C. cellulosae.


Fig. 2
SDS-PAGE/EITB findings in 24 patients sera of neurocysticercosis to cystic fluid of C. cellulosae. Mr: molecular weight in kDa, A: SDS-PAGE of cystic fluid (lane 3 in Fig. 1), 1~24: sera from patients.


Fig. 3
SDS-PAGE/EITB findings in 24 patients sera of neurocysticercosis to saline extract of scolex of C. cellulosae. Mr: molecular weight in kDa, A: SDS-PAGE of the saline extract (lane 7, Fig. 1), 1~24: patients sera.


Fig. 4
SDS-PAGE/EITB findings in 24 patients sera of neurocysticercosis to saline extract of whole worm of C. cellulosae. Mr: molecular weight in kDa, A: SDS-PAGE of the saline extract (lane 8, Fig. 1), 1~24: patients sera.


Fig. 5
SDS-PAGE/EITB findings in 24 patients sera of neurocysticercosis to saline extract of sparganum. Mr: molecular weight in kDa, A: SDS-PAGE of the saline extract (lane 1 in Fig. 1), 1~24: patients sera.


Fig. 6
SDS-PAGE/EITB findings in 24 patients sera of neurocysticercosis to hydatid cyst fluid. Mr: molecular weight in kDa, A: SDS-PAGE of hydatid cyst fluid (lane 2 in Fig. 1), 1~24: patient sera.


Fig. 7
SDS-PAGE/EITB findings of a monoclonal antibody to antigens of C. cellulosae. Mr: molecular weight in kDa, 1: cystic fluid, 2: saline extract of scolex.

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