Studies on lactic dehydrogenase activity in parasitic helminths

, Lee, Soon Hyung

doi:1967.5.1.5

Korean J Parasito > Volume 5(1):1967 > Article

Original Article


Korean J Parasitol. 1967 Jun;5(1):5-16. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1967.5.1.5
Copyright © 1967 by The Korean Society for Parasitology


Studies on lactic dehydrogenase activity in parasitic helminths
Soon Hyung Lee
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.


Abstract
A series of experiments was performed to determine the lactic dehydrogenase activity of various parasitic helminths. The enzyme activity was determined by the modified method of Wroblewshi and LaDue (1955) using tissue homogenate of 16 kinds of worm parasites. The worms were mostly collected alive from local abattoir and removed from the organ or tissues of the naturally infected animal host and some materials were also obtained from the human host. They were thoroughly washed and homogenized in chilled glass tissue grinder, and then centrifuged. The supernatants were designated as enzyme preparations, and their enzyme activity was measured by spectrophotometry at the wave length of 340 millimicron. In order to know the effects of temperature and substrate concentration on the enzyme activity, the extinction of reduced Coenzyme I(NADH) was measured at the various conditions of incubation temperature and substrate concentration. The results of this experiments were as follows: 1. The lactic dehydrogenase activity occurred over all kinds of parasites used in this study. 2. Most worms of nematodes and trematodes displayed their maximum activity in the range of pH 2.7~3.5, and cestodes revealed their maximum activity in the ranges of both pH 2.7~3.5 and pH 7.4. 3. In nematodes and trematodes, the lactic dehydrogenase activity increased slowly as incubation temperature increases except in the case of Eurytrema pancreaticum, while the activity in cestodes decreased inversely. 4. The lactic dehydrogenase activity increased in proportion to the increase of substrate concentration in most of worm parasites.

Figures


	Fig. 1 LDH activity in 3 kinds of Nematodes.


	Fig. 2 LDH activity in 5 kinds of Trematodes.


	Fig. 3 LDH activity in 5 kinds of cestodes


	Fig. 4 LDH activity in Taenia solium


	Fig. 5 LDH activity in Taenia pisiformis


	Fig. 6 LDH activity in Sparganum and Diphyllobothrium mansoni


	Fig. 7 Relation between LDH activity and incubation temperature in Nematodes


	Fig. 8 Relation between LDH activity and incubation temperature in Trematodes


	Fig. 9 Relation between LDH activity and incubation temperature in Cestodes.


	Fig. 10 Effects of the substrate concentration on the LDH activity in Nematodes


	Fig. 11 Effects of the substrate concentration on the LDH activity in Trematodes


	Fig. 12 Effects of the substrate concentration on the LDH activity in Cestodes

Tables


	Table 1 Lactic Dehydrogenase Activity in Various Helminths. (Changes of optical density per milligram nitrogen of worm tissue per minute)


	Table 2 Optimal pH of LDH Activity


	Table 3 Effects of the Incubation Temperature on the Activity of LDH (Changes of O.D./mgN/min)


	Table 4 Substrate Concentration and Lactic Dehydrogenase Activity (Changes of O.D./mgN/min)

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