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Korean J Parasitol > Volume 2(3):1964 > Article

Original Article
Korean J Parasitol. 1964 Dec;2(3):170-174. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1964.2.3.170
Copyright © 1964 by The Korean Society for Parasitology
Metabolism of C14-glucose by Fasciola hepatica
Byong Seol Seo,Han Jong Rim,Kwang Soo Kim,Sang Don Rhee,Sun Jo Lim and Wha Suk Lee
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Department of Physiology, College of Medicine, Seoul National University, Korea.
Abstract

1. The glucose uptake rate by Fasciola hepatica was a mean value of 9.62 ± 0.54 µM/hr/g, and total CO2 production rate by the flukes averaged 24.28 ± 4.26 µM/hr/g wet wt. The relative specific activity of respiratory CO2 showed a mean value of 79.89 ± 1.78 per cent. The rate of CO2 production derived from medium C14-glucose was a mean of 19.55 ± 3.56 µM/hr/g of we wt. Therefore, the average value of 32.72 ± 4.8 percent of glucose utilized by the flukes from the medium C14-glucose was oxidized to respiratory CO2.

2. The tissue concentration of glycogen in F. hepatica was a mean of 38.36 ± 2.91 mg/g or 3.84 ± 0.29 %/g of wet wt, and the turnover rate of glycogen pool was a mean of 1.6±0.22 %/hr or 0.65 ± 0.13 mg/hr/g. The average value of 37.26 ± 3.86 per cent of glucose utilized by the fluke from the medium C14-glucose was incorporated to the glycogen.

3. These data account for that approximately 70 per cent of the utilized glucose by the flukes participated in furnishing the oxidation into respiratory CO2 and the synthetic process into glycogen.

Tables


Table 1
The glucose uptake rate and conversion of C14-glucose into respiratory CO2.


Table 2
The tissue concentration of glycogen and conversion of C14-glucose into glycogen.

References
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