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Korean J Parasitol > Volume 20(1):1982 > Article

Original Article
Korean J Parasitol. 1982 Jun;20(1):9-13. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1982.20.1.9
Copyright © 1982 by The Korean Society for Parasitology
Localization and isolation of anthelmintic activity on Clonorchis sinensis from stem cortex of Machilis thunbergii
Jae Ku Rhee,Byeong Kirl Baek,Byung Zun Ahn and Sung Hwan Oh
Department of Veterinary Medicine, Jeonbug National University, Korea.
Department of Pharmacy, Chungnam National University, Korea.
Oriental Medical College, Wonkwang University, Korea.
Abstract

The present work was to localize and isolate the anthelmintic substance from stem cortex of Machilis thunbergii which grows in all parts of the Korea peninsula. The dried M. thunbergii bark was extracted with a solvent mixture (Methanol: Ethyl acetate/1:1) by a Soxhlet extractor at 70C for 24 hours, and then the extract was separated into solid matter (negative wormicidal) and benzene extract (positive wormicidal) by benzene solvent. The anthelmintic action was observed on the cercaria, the liberated metacercaria and the adult of Clonorchis sinensis.

On the silica gel thin layer chromatography with a spot of benzene extract in benzene solvent, nine spots were observed in iodine chamber and the spot of Rf. 0.16 value of them appeared the most strong wormicidal effect on the cercaria and the liberated metacercaria of C. sinensis.

Afterward, a 0.3 g colourless solid substance which was equivalent to the Rf. 0.16 value spot was isolated from the benzene extract of 3 kg M. thunbergii stem cortex by means of silica gel column chromatography using benzene carrier, and the adult of C. sinensis was killed within 6 minutes by 0.05 percent solution of the substance.

Figures


Fig. 1
Localization and isolation method of wormicidal substance from Machilus thunbergii.

*: Wormicidal activity


Tables


Table 1
Wormicidal effect of MeOH and ethyl acetate extracts of Machilus thunbergii on Clonorchis sinensis cercaria


Table 2
Wormicidal effect of MeOH and ethyl acetate extracts of Machilus thunbergii on liberated metacercaria of Clonorchis sinensis


Table 3
Wormicidal effect of MeOH and ethyl acetate extracts of Machilus thunbergii on the adult of Clonorchis sinensis

References
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4. Rhee JK, Baek BK, Ahn BZ, Park YJ. [The Wormicidal Substances Of Fresh Water Fishes On Clonorchis Sinensis: II. Preliminary Research On The Wormicidal Substances From Mucous Substances Of Various Fresh Water Fishes]. Korean J Parasitol 1980;18(1):98–104.
 
5. Rhee JK, Woo KJ, Baek BK, Ahn BJ. Screening of the wormicidal Chinese raw drugs on Clonorchis sinensis. Am J Chin Med 1981;9(4):277–284.
  
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